Fate of organic nitrogen in amino acids during alternating denitrification and nitrification

IF 4.1 2区 环境科学与生态学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Haiyun Zhang , Yue Ma , Xu Peng , Mo Li , Yongming Zhang , Bruce E. Rittmann
{"title":"Fate of organic nitrogen in amino acids during alternating denitrification and nitrification","authors":"Haiyun Zhang ,&nbsp;Yue Ma ,&nbsp;Xu Peng ,&nbsp;Mo Li ,&nbsp;Yongming Zhang ,&nbsp;Bruce E. Rittmann","doi":"10.1016/j.ibiod.2024.105850","DOIUrl":null,"url":null,"abstract":"<div><p>Nitrogen-containing organic compounds, such as amino acids in soybean-processing wastewater, can be used as electron donors to drive denitrification, but their biodegradation releases ammonium nitrogen that must be nitrified and denitrified to maintain total-nitrogen removal. We evaluated glutamate, isoleucine, and methionine as example amino acids to explore the fate of nitrogen when they are used as electron donor to drive denitrification during two stages of alternating denitrification and nitrification. The experimental results documented that each amino acid enabled complete removal of exogenous NO<sub>3</sub><sup>−</sup> in the first stage of denitrification and complete NO<sub>3</sub><sup>−</sup> removal in the second stage. After two alternations of denitrification and nitrification, the TN concentration in effluent was less than 5 mgN/L for all amino acids, and COD in the effluent was less than 25 mg/L. Based on stoichiometry and the ratio of chemical oxygen demand (COD) to organic N in each amino acid, 57%–66% of the COD from the amino acids had to be oxidized to reduce the endogenous NO<sub>3</sub><sup>−</sup>–N in the first stage. N from the amino acids was nitrified and denitrified in the subsequent nitrification and denitrification stages, and the percentages of COD used for denitrification from both stages were 72%–85%. The residual NH<sub>4</sub><sup>+</sup>-N concentrations were slightly higher with methionine, possibly due to inhibition from sulfide released from methionine.</p></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"193 ","pages":"Article 105850"},"PeriodicalIF":4.1000,"publicationDate":"2024-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Biodeterioration & Biodegradation","FirstCategoryId":"93","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0964830524001215","RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

Nitrogen-containing organic compounds, such as amino acids in soybean-processing wastewater, can be used as electron donors to drive denitrification, but their biodegradation releases ammonium nitrogen that must be nitrified and denitrified to maintain total-nitrogen removal. We evaluated glutamate, isoleucine, and methionine as example amino acids to explore the fate of nitrogen when they are used as electron donor to drive denitrification during two stages of alternating denitrification and nitrification. The experimental results documented that each amino acid enabled complete removal of exogenous NO3 in the first stage of denitrification and complete NO3 removal in the second stage. After two alternations of denitrification and nitrification, the TN concentration in effluent was less than 5 mgN/L for all amino acids, and COD in the effluent was less than 25 mg/L. Based on stoichiometry and the ratio of chemical oxygen demand (COD) to organic N in each amino acid, 57%–66% of the COD from the amino acids had to be oxidized to reduce the endogenous NO3–N in the first stage. N from the amino acids was nitrified and denitrified in the subsequent nitrification and denitrification stages, and the percentages of COD used for denitrification from both stages were 72%–85%. The residual NH4+-N concentrations were slightly higher with methionine, possibly due to inhibition from sulfide released from methionine.

Abstract Image

反硝化和硝化交替过程中氨基酸中有机氮的去向
含氮有机化合物(如大豆加工废水中的氨基酸)可用作电子供体来驱动反硝化,但它们的生物降解会释放出铵态氮,而铵态氮必须经过硝化和反硝化才能维持总氮去除率。我们以谷氨酸、异亮氨酸和蛋氨酸为例,探讨了在反硝化和硝化交替进行的两个阶段中,当它们作为电子供体驱动反硝化时氮的去向。实验结果表明,每种氨基酸都能在第一阶段反硝化过程中完全去除外源 NO3-,并在第二阶段完全去除 NO3-。经过两次反硝化和硝化交替后,所有氨基酸的出水 TN 浓度均小于 5 mgN/L,出水 COD 小于 25 mg/L。根据化学计量学和每种氨基酸中化学需氧量(COD)与有机氮的比例,氨基酸中57%-66%的COD必须在第一阶段被氧化,以减少内源NO3--N。氨基酸中的氮在随后的硝化和反硝化阶段被硝化和反硝化,这两个阶段用于反硝化的 COD 百分比为 72%-85%。蛋氨酸的残余 NH4+-N 浓度略高,这可能是由于蛋氨酸释放的硫化物的抑制作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
CiteScore
9.60
自引率
10.40%
发文量
107
审稿时长
21 days
期刊介绍: International Biodeterioration and Biodegradation publishes original research papers and reviews on the biological causes of deterioration or degradation.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信