Exploring butyrylcholinesterase expression in living cells and diabetic mouse models using a fluorescent probe with near-infrared excitation and emission maxima

IF 4.1 3区工程技术 Q2 CHEMISTRY, APPLIED

Dyes and Pigments Pub Date : 2024-06-18 DOI:10.1016/j.dyepig.2024.112295

Bin Yang , Yujia He , Xiangdong Ding , Zhongling Liu , Xinghua Wang , Wei Yu

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Abstract

Butyrylcholinesterase (BChE), as a member of the cholinesterase family, is widely expressed in various tissues and cell types. Abnormal activity levels of BChE in organ systems or cells are usually associated with diseases. Therefore, developing effective methods for monitoring the distribution and activity of BChE in vivo is significant for exploring the physiological effects of BChE in organisms and improving prophylaxis, diagnosis or therapy of diseases. In this study, a dihydroxanthene-derived fluorescent probe CHQ-B with near-infrared (NIR) excitation and emission maxima was developed for visualizing endogenous BChE in vivo. This probe exhibits superior selectivity and sensitivity towards BChE, as well as low cytotoxicity and good biocompatibility to biosamples. Furthermore, efficient and robust fluorescence imaging of the distribution and activity of BChE in diabetic mouse models confirmed the high expression of BChE induced by diabetes. Results of experiments demonstrated that CHQ-B is a promising fluorescence imaging tool for exploring butyrylcholinesterase expression in organisms during researches of relationships between BChE expression and various diseases.

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利用具有近红外激发和发射最大值的荧光探针探索丁酰胆碱酯酶在活细胞和糖尿病小鼠模型中的表达情况

丁酰胆碱酯酶（BChE）是胆碱酯酶家族的一员，在各种组织和细胞类型中广泛表达。器官系统或细胞中 BChE 活性水平异常通常与疾病有关。因此，开发有效的方法来监测 BChE 在体内的分布和活性，对于探索 BChE 在生物体内的生理效应以及改善疾病的预防、诊断或治疗具有重要意义。本研究开发了一种二羟基蒽衍生的荧光探针 CHQ-B，它具有近红外（NIR）激发和发射最大值，可用于体内内源性 BChE 的可视化。该探针对 BChE 具有卓越的选择性和灵敏度，而且细胞毒性低，对生物样品具有良好的生物相容性。此外，对糖尿病小鼠模型中 BChE 的分布和活性进行高效、稳健的荧光成像证实了糖尿病诱导的 BChE 的高表达。实验结果表明，在研究 BChE 表达与各种疾病的关系时，CHQ-B 是一种很有前途的荧光成像工具，可用于探索丁酰胆碱酯酶在生物体内的表达。

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来源期刊

Dyes and Pigments 工程技术-材料科学：纺织

CiteScore

8.20

自引率

13.30%

发文量

933

审稿时长

33 days

期刊介绍： Dyes and Pigments covers the scientific and technical aspects of the chemistry and physics of dyes, pigments and their intermediates. Emphasis is placed on the properties of the colouring matters themselves rather than on their applications or the system in which they may be applied. Thus the journal accepts research and review papers on the synthesis of dyes, pigments and intermediates, their physical or chemical properties, e.g. spectroscopic, surface, solution or solid state characteristics, the physical aspects of their preparation, e.g. precipitation, nucleation and growth, crystal formation, liquid crystalline characteristics, their photochemical, ecological or biological properties and the relationship between colour and chemical constitution. However, papers are considered which deal with the more fundamental aspects of colourant application and of the interactions of colourants with substrates or media. The journal will interest a wide variety of workers in a range of disciplines whose work involves dyes, pigments and their intermediates, and provides a platform for investigators with common interests but diverse fields of activity such as cosmetics, reprographics, dye and pigment synthesis, medical research, polymers, etc.

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