Enhancement of serrawettin W1-producing Serratia marcescens cell migration by resonant oscillation under alternating current electric field

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal Pub Date : 2024-06-21 DOI:10.1016/j.bej.2024.109407

Yukiya Kobayashi , Yuki Ayusawa , Mizuki Yamaguchi , Sumihiro Koyama , Yoichi Ishikawa , Eri Nasuno , Norihiro Kato

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引用次数: 0

Abstract

Swarming migration is observed in flagellated bacteria on wet surfaces. As the secreted biosurfactant hydrates, friction between the cell and the wet surface is reduced, and weak flagellar movement appears to be a significant force for cell translocation. Developing an artificial swarming control method could greatly aid in establishing techniques for biofilm inhibition and detachment. In this study, the effect of forced cell vibration by an alternating current electric field (ACEF) on swarming motility was investigated using the serrawettin W1-producing Serratia marcescens strain. At frequencies close to the natural frequencies of microbial cells (12 MHz), swarming motion was effectively enhanced in biosurfactant-producing S. marcescens, but not in non-surfactant-producing Escherichia coli or non-flagellated Staphylococcus aureus. Electric field-assisted cell migration was significantly induced under swarming conditions with low friction resistance between the cell and gel surface. This finding suggests a direction for developing strategies to regulate biofilm formation and detachment using ACEF-assisted oscillation of cells attached to surfaces.

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交变电场下的共振振荡增强了产丝兰素 W1 的 Serratia marcescens 细胞迁移能力

在潮湿表面上观察到鞭毛细菌的蜂拥迁移。随着分泌的生物表面活性剂水化，细胞与潮湿表面之间的摩擦力减小，微弱的鞭毛运动似乎成为细胞迁移的重要力量。开发一种人工蜂群控制方法对建立生物膜抑制和脱离技术大有帮助。在本研究中，使用产生丝拉韦汀 W1 的 Serratia marcescens 菌株研究了交流电场（ACEF）强迫细胞振动对蜂群运动的影响。在接近微生物细胞固有频率（12 兆赫）的频率下，产生生物表面活性剂的 S. marcescens 菌的蜂拥运动得到了有效增强，而不产生表面活性剂的大肠杆菌或无鞭毛的金黄色葡萄球菌则没有这种效果。在细胞与凝胶表面之间摩擦阻力较低的蜂拥条件下，电场辅助细胞迁移有明显的诱导作用。这一发现为利用 ACEF 辅助附着在表面上的细胞摆动来调节生物膜的形成和脱落提供了一个发展方向。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biochemical Engineering Journal 工程技术-工程：化工

CiteScore

7.10

自引率

5.10%

发文量

380

审稿时长

34 days

期刊介绍： The Biochemical Engineering Journal aims to promote progress in the crucial chemical engineering aspects of the development of biological processes associated with everything from raw materials preparation to product recovery relevant to industries as diverse as medical/healthcare, industrial biotechnology, and environmental biotechnology. The Journal welcomes full length original research papers, short communications, and review papers* in the following research fields: Biocatalysis (enzyme or microbial) and biotransformations, including immobilized biocatalyst preparation and kinetics Biosensors and Biodevices including biofabrication and novel fuel cell development Bioseparations including scale-up and protein refolding/renaturation Environmental Bioengineering including bioconversion, bioremediation, and microbial fuel cells Bioreactor Systems including characterization, optimization and scale-up Bioresources and Biorefinery Engineering including biomass conversion, biofuels, bioenergy, and optimization Industrial Biotechnology including specialty chemicals, platform chemicals and neutraceuticals Biomaterials and Tissue Engineering including bioartificial organs, cell encapsulation, and controlled release Cell Culture Engineering (plant, animal or insect cells) including viral vectors, monoclonal antibodies, recombinant proteins, vaccines, and secondary metabolites Cell Therapies and Stem Cells including pluripotent, mesenchymal and hematopoietic stem cells; immunotherapies; tissue-specific differentiation; and cryopreservation Metabolic Engineering, Systems and Synthetic Biology including OMICS, bioinformatics, in silico biology, and metabolic flux analysis Protein Engineering including enzyme engineering and directed evolution.