{"title":"Selenomethionine-Conjugated Extracellular Vesicles for ROS-Mediated Cell Apoptosis","authors":"Siyu Li, Zhaorong Ouyang, Mengjie Zhang, Shuai Guo, Biao Cai* and Houli Liu*, ","doi":"10.1021/acsanm.4c01793","DOIUrl":null,"url":null,"abstract":"<p >Selenomethionine (SeM) holds great potential applications in tumor therapy. However, the tumor-targeting ability of SeM <i>in vivo</i> remains challenging. Herein, we utilize extracellular vesicles (EV) as tumor-targeted drug delivery systems to achieve enhanced specific targeting and antitumor efficacy. The carboxyl groups of SeM are conjugated with the amino groups of EV derived from low-pH culture medium reprogrammed CT26 cells (LEV) to obtain the SeM-based formulations (SMLEV), which can actively target tumor cells and enhance uptake efficacy through specific behaviors of LEV to their parent cells. Mechanistic studies indicate that SMLEV can induce reactive oxygen species (ROS) overproduction, mitochondrial dysfunction, as well as Caspase-9 and Caspase-3 activation. Here, SMLEV exhibit enhanced cytotoxic potential toward colon tumor (CT26) cells. After systemic administration, the growth of tumors is inhibited <i>in vivo</i> using CT26 tumor-bearing mice. Our findings can provide insights and a strategy in developing SeM delivery for tumor treatment.</p>","PeriodicalId":6,"journal":{"name":"ACS Applied Nano Materials","volume":null,"pages":null},"PeriodicalIF":5.3000,"publicationDate":"2024-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"ACS Applied Nano Materials","FirstCategoryId":"88","ListUrlMain":"https://pubs.acs.org/doi/10.1021/acsanm.4c01793","RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MATERIALS SCIENCE, MULTIDISCIPLINARY","Score":null,"Total":0}
引用次数: 0
Abstract
Selenomethionine (SeM) holds great potential applications in tumor therapy. However, the tumor-targeting ability of SeM in vivo remains challenging. Herein, we utilize extracellular vesicles (EV) as tumor-targeted drug delivery systems to achieve enhanced specific targeting and antitumor efficacy. The carboxyl groups of SeM are conjugated with the amino groups of EV derived from low-pH culture medium reprogrammed CT26 cells (LEV) to obtain the SeM-based formulations (SMLEV), which can actively target tumor cells and enhance uptake efficacy through specific behaviors of LEV to their parent cells. Mechanistic studies indicate that SMLEV can induce reactive oxygen species (ROS) overproduction, mitochondrial dysfunction, as well as Caspase-9 and Caspase-3 activation. Here, SMLEV exhibit enhanced cytotoxic potential toward colon tumor (CT26) cells. After systemic administration, the growth of tumors is inhibited in vivo using CT26 tumor-bearing mice. Our findings can provide insights and a strategy in developing SeM delivery for tumor treatment.
期刊介绍:
ACS Applied Nano Materials is an interdisciplinary journal publishing original research covering all aspects of engineering, chemistry, physics and biology relevant to applications of nanomaterials. The journal is devoted to reports of new and original experimental and theoretical research of an applied nature that integrate knowledge in the areas of materials, engineering, physics, bioscience, and chemistry into important applications of nanomaterials.