Antibacterial, Antibiofilm, and Tooth Color Preservation Capacity of Magnesium Oxide Nanoparticles Varnish (in vitro Study).

IF 4.9 Q2 NANOSCIENCE & NANOTECHNOLOGY
Nanotechnology, Science and Applications Pub Date : 2024-06-27 eCollection Date: 2024-01-01 DOI:10.2147/NSA.S462771
Sonya Jamal Hamalaw, Fadil Abdulla Kareem, Arass Jalal Noori
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引用次数: 0

Abstract

Purpose: Antibacterial and antibiofilm properties of magnesium oxide nanoparticles (MgONPs) mixture assessed against Streptococcus mutans (S. mutans), in addition to examining MgONPs varnish impact on the preservation of the tooth color and inhibition of methylene blue diffusion to the enamel.

Methods: MgONPs mixture was prepared in deionized water (DW), absolute ethanol (E), and rosin with ethanol (RE), named varnish. The antibacterial and antibiofilm capacities of MgONPs mixtures were tested by agar well diffusion, colony-forming unit (CFU), and biofilm inhibition microtiter methods in triplicate and compared to sodium fluoride varnish (NaF) and chlorhexidine mouthwash (ChX). A spectrophotometer was used to record basic tooth color. The artificial demineralization was initiated for 96 h. Then, experimental materials were applied to the corresponding group, and 10-day pH cycles proceeded. Then, the color was recorded in the same ambient environment. The methylene blue diffusion was evaluated by staining the samples for 24 h. After that, the diffusion test was calculated by a digital camera attached to the stereomicroscope.

Results: The agar well diffusion test expressed a significant inhibition zone with all MgONPs mixtures (p = 0.000), and maximum inhibition zone diameter associated with MgONPs-RE. The same finding was observed in the CFU test. Additionally, 2.5%, 5%, and 10% MgONPs-RE varnish showed strong biofilm inhibition capacity (p = 0.039) compared to NaF and ChX groups that inhibit biofilm formation moderately (p = 0.003). The study shows that the 5% MgONPs-RE varnish maintains basic tooth color with minimal methylene blue diffusion compared to NaF varnish (p = 0.00).

Conclusion: Evaluating MgONPs as a mixture revealed antibacterial and antibiofilm capacity against S. mutans with a higher effect of MgONPs-RE varnish. Also, examining the topical effect of MgONPs-RE varnish on the preservation of the tooth color after pH cycle challenges and methylene blue diffusion to enamel confirmed the high performance of MgONPs-RE varnish at 5%.

氧化镁纳米粒子涂膜的抗菌、抗生物膜和牙齿保色能力(体外研究)。
目的:评估氧化镁纳米粒子(MgONPs)混合物对变异链球菌(S. mutans)的抗菌和抗生物膜特性,此外还研究 MgONPs 光油对保持牙齿颜色和抑制亚甲蓝向珐琅质扩散的影响:方法:在去离子水(DW)、绝对乙醇(E)和含乙醇的松香(RE)中制备 MgONPs 混合物,命名为清漆。采用琼脂井扩散法、菌落形成单位法和生物膜抑制微滴定法测试了 MgONPs 混合物的抗菌和抗生物膜能力,一式三份,并与氟化钠清漆(NaF)和洗必泰漱口水(ChX)进行了比较。使用分光光度计记录牙齿的基本颜色。然后将实验材料涂抹在相应的组别上,进行为期 10 天的 pH 循环。然后,在相同的环境中记录牙色。将样品染色 24 小时,评估亚甲基蓝扩散情况:结果:琼脂井扩散试验表明,所有 MgONPs 混合物都有明显的抑制区(p = 0.000),MgONPs-RE 的抑制区直径最大。在 CFU 试验中也观察到了同样的结果。此外,与 NaF 和 ChX 组相比,2.5%、5% 和 10%的 MgONPs-RE 清漆显示出很强的生物膜抑制能力(p = 0.039),而 NaF 和 ChX 组对生物膜形成的抑制作用一般(p = 0.003)。研究表明,与 NaF 光油相比,5% MgONPs-RE 光油可保持基本牙色,亚甲蓝扩散极小(p = 0.00):将 MgONPs 作为混合物进行评估后发现,MgONPs-RE 上光剂具有更强的抗菌和抗生物膜能力。此外,通过研究 MgONPs-RE 清漆在 pH 循环挑战和亚甲蓝向珐琅质扩散后对牙齿颜色保持的局部效果,证实了 5% 的 MgONPs-RE 清漆具有很高的性能。
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来源期刊
Nanotechnology, Science and Applications
Nanotechnology, Science and Applications NANOSCIENCE & NANOTECHNOLOGY-
CiteScore
11.70
自引率
0.00%
发文量
3
审稿时长
16 weeks
期刊介绍: Nanotechnology, Science and Applications is an international, peer-reviewed, Open Access journal that focuses on the science of nanotechnology in a wide range of industrial and academic applications. The journal is characterized by the rapid reporting of reviews, original research, and application studies across all sectors, including engineering, optics, bio-medicine, cosmetics, textiles, resource sustainability and science. Applied research into nano-materials, particles, nano-structures and fabrication, diagnostics and analytics, drug delivery and toxicology constitute the primary direction of the journal.
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