High-yield nanovesicles extruded from dental follicle stem cells promote the regeneration of periodontal tissues as an alternative of exosomes

IF 5.8 1区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Clinical Periodontology Pub Date : 2024-07-01 DOI:10.1111/jcpe.14036

Lu Liang, Limeiting Wang, Zhenhui Liao, Liya Ma, Pinwen Wang, Junjie Zhao, Jinyan Wu, Hefeng Yang

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引用次数: 0

Abstract

Aim

To identify an optimized strategy for the large-scale production of nanovesicles (NVs) that preserve the biological properties of exosomes (EXOs) for use in periodontal regeneration.

Materials and Methods

NVs from dental follicle stem cells (DFSCs) were prepared through extrusion, and EXOs from DFSCs were isolated. The yield of both extruded NVs (eNVs) and EXOs were quantified through protein concentration and particle number analyses. Their pro-migration, pro-proliferation and pro-osteogenesis capacities were compared subsequently in vitro. Additionally, proteomics analysis was conducted. To further evaluate the periodontal regeneration potential of eNVs and EXOs, they were incorporated into collagen sponges and transplanted into periodontal defects in rats. In vivo imaging and H&E staining were utilized to verify their biodistribution and safety. Micro-Computed Tomography analysis and histological staining were performed to examine the regeneration of periodontal tissues.

Results

The yield of eNVs was nearly 40 times higher than that of EXOs. Interestingly, in vitro experiments indicated that the pro-migration and pro-proliferation abilities of eNVs were superior, and the pro-osteogenesis potential was comparable to EXOs. More importantly, eNVs exhibited periodontal regenerative potential similar to that of EXOs.

Conclusions

Extrusion has proven to be an efficient method for generating numerous eNVs with the potential to replace EXOs in periodontal regeneration.

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牙泡干细胞挤出的高产纳米颗粒作为外泌体的替代物促进了牙周组织的再生。

目的：确定大规模生产保持外泌体（EXOs）生物特性的纳米颗粒（NVs）的优化策略，以用于牙周再生：材料：通过挤压法制备来自牙泡干细胞（DFSCs）的NVs，并从DFSCs中分离出EXOs。通过蛋白质浓度和颗粒数分析，对挤压NVs（eNVs）和EXOs的产量进行了量化。随后在体外比较了它们的促迁移、促增殖和促骨质生成能力。此外，还进行了蛋白质组学分析。为了进一步评估 eNVs 和 EXOs 的牙周再生潜力，将它们纳入胶原海绵并移植到大鼠的牙周缺损处。利用体内成像和 H&E 染色来验证它们的生物分布和安全性。显微计算机断层扫描分析和组织学染色检查了牙周组织的再生情况：结果：eNVs 的产量比 EXOs 高近 40 倍。有趣的是，体外实验表明，eNVs 的促迁移和促增殖能力更强，其促成骨潜力与 EXOs 相当。更重要的是，eNVs 表现出与 EXOs 相似的牙周再生潜力：结论：事实证明，挤压是生成大量 eNVs 的有效方法，具有取代 EXOs 进行牙周再生的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Clinical Periodontology 医学-牙科与口腔外科

CiteScore

13.30

自引率

10.40%

发文量

175

审稿时长

3-8 weeks

期刊介绍： Journal of Clinical Periodontology was founded by the British, Dutch, French, German, Scandinavian, and Swiss Societies of Periodontology. The aim of the Journal of Clinical Periodontology is to provide the platform for exchange of scientific and clinical progress in the field of Periodontology and allied disciplines, and to do so at the highest possible level. The Journal also aims to facilitate the application of new scientific knowledge to the daily practice of the concerned disciplines and addresses both practicing clinicians and academics. The Journal is the official publication of the European Federation of Periodontology but wishes to retain its international scope. The Journal publishes original contributions of high scientific merit in the fields of periodontology and implant dentistry. Its scope encompasses the physiology and pathology of the periodontium, the tissue integration of dental implants, the biology and the modulation of periodontal and alveolar bone healing and regeneration, diagnosis, epidemiology, prevention and therapy of periodontal disease, the clinical aspects of tooth replacement with dental implants, and the comprehensive rehabilitation of the periodontal patient. Review articles by experts on new developments in basic and applied periodontal science and associated dental disciplines, advances in periodontal or implant techniques and procedures, and case reports which illustrate important new information are also welcome.