The Potential Significance of the EMILIN3 Gene in Augmenting the Aggressiveness of Low-Grade Gliomas is Noteworthy.

IF 2.5 4区医学 Q3 ONCOLOGY

Cancer Management and Research Pub Date : 2024-06-26 eCollection Date: 2024-01-01 DOI:10.2147/CMAR.S463694

Li Ao Wang, Zhiming Zheng, Jia Zheng, Guifeng Zhang, Zheng Wang

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引用次数: 0

Abstract

Purpose: Low-grade gliomas (LGG) are common brain tumors with high mortality rates. Cancer cell invasion is a significant factor in tumor metastasis. Novel biomarkers are urgently needed to predict LGG prognosis effectively.

Methods: The data for LGG were obtained from the Bioinformatics database. A consensus clustering analysis was performed to identify molecular subtypes linked with invasion in LGG. Differential expression analysis was performed to identify differentially expressed genes (DEGs) between the identified clusters. Enrichment analyses were then conducted to explore the function for DEGs. Prognostic signatures were placed, and their predictive power was assessed. Furthermore, the invasion-related prognostic signature was validated using the CGGA dataset. Subsequently, clinical specimens were procured in order to validate the expression levels of the distinct genes examined in this research, and to further explore the impact of these genes on the glioma cell line LN229 and HS-683.

Results: Two invasion-related molecular subtypes of LGG were identified, and we sifted 163 DEGs between them. The enrichment analyses indicated that DEGs are mainly related to pattern specification process. Subsequently, 10 signature genes (IGF2BP2, SRY, CHI3L1, IGF2BP3, MEOX2, ABCC3, HOXC4, OTP, METTL7B, and EMILIN3) were sifted out to construct a risk model. Besides, the survival (OS) in the high-risk group was lower. The performance of the risk model was verified. Furthermore, a highly reliable nomogram was generated. Cellular experiments revealed the ability to promote cell viability, value-addedness, migratory ability, invasive ability, and colony-forming ability of the glioma cell line LN229 and HS-683. The qRT-PCR analysis of clinical glioma samples showed that these 10 genes were expressed at higher levels in high-grade gliomas than in low-grade gliomas, suggesting that these genes are associated with poor prognosis of gliomas.

Conclusion: Our study sifted out ten invasion-related biomarkers of LGG, providing a reference for treatments and prognostic prediction in LGG.

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EMILIN3 基因在增强低级别胶质瘤侵袭性方面的潜在意义值得关注。

目的：低级别胶质瘤（LGG）是死亡率很高的常见脑肿瘤。癌细胞侵袭是肿瘤转移的一个重要因素。目前迫切需要新型生物标志物来有效预测低级别胶质瘤的预后：方法：LGG 的数据来自生物信息学数据库。方法：从生物信息学数据库中获取LGG数据，进行共识聚类分析，以确定与LGG侵袭相关的分子亚型。进行差异表达分析，以确定已识别聚类之间的差异表达基因（DEGs）。然后进行了富集分析，以探索 DEGs 的功能。建立了预后特征，并对其预测能力进行了评估。此外，还利用 CGGA 数据集验证了与侵袭相关的预后特征。随后，采集了临床标本，以验证本研究中考察的不同基因的表达水平，并进一步探讨这些基因对胶质瘤细胞系 LN229 和 HS-683 的影响：结果：我们发现了两种与侵袭相关的 LGG 分子亚型，并筛选出了它们之间的 163 个 DEGs。富集分析表明，DEGs主要与模式规范过程有关。随后，我们筛选出10个特征基因（IGF2BP2、SRY、CHI3L1、IGF2BP3、MEOX2、ABCC3、HOXC4、OTP、METTL7B和EMILIN3），构建了一个风险模型。此外，高风险组的生存率（OS）较低。风险模型的性能得到了验证。此外，还生成了一个高度可靠的提名图。细胞实验显示，该药物能促进胶质瘤细胞株 LN229 和 HS-683 的细胞活力、增值能力、迁移能力、侵袭能力和集落形成能力。对临床胶质瘤样本进行的qRT-PCR分析表明，这10个基因在高级别胶质瘤中的表达水平高于低级别胶质瘤，这表明这些基因与胶质瘤的不良预后有关：我们的研究筛选出了10个与LGG侵袭相关的生物标志物，为LGG的治疗和预后预测提供了参考。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cancer Management and Research Medicine-Oncology

CiteScore

7.40

自引率

0.00%

发文量

448

审稿时长

16 weeks

期刊介绍： Cancer Management and Research is an international, peer reviewed, open access journal focusing on cancer research and the optimal use of preventative and integrated treatment interventions to achieve improved outcomes, enhanced survival, and quality of life for cancer patients. Specific topics covered in the journal include: ◦Epidemiology, detection and screening ◦Cellular research and biomarkers ◦Identification of biotargets and agents with novel mechanisms of action ◦Optimal clinical use of existing anticancer agents, including combination therapies ◦Radiation and surgery ◦Palliative care ◦Patient adherence, quality of life, satisfaction The journal welcomes submitted papers covering original research, basic science, clinical & epidemiological studies, reviews & evaluations, guidelines, expert opinion and commentary, and case series that shed novel insights on a disease or disease subtype.