First isolation and whole-genome sequencing of Trueperella abortisuis from a goat in Canada.

IF 0.9 4区农林科学 Q3 VETERINARY SCIENCES

Canadian Veterinary Journal-revue Veterinaire Canadienne Pub Date : 2024-07-01

Kazal Ghosh, Glenna McGregor, Giselle Hughes, Michael Trimble, William Hsiao, Anatoliy Trokhymchuk

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引用次数: 0

Abstract

Objective: The present study reports the first isolation and whole-genome sequencing of a Trueperella abortisuis bacterium from a goat.

Animals and sample: The T. abortisuis was isolated from the uterus of a goat following an abortion.

Procedure: The T. abortisuis was identified by pure culture phenotype and MALDI-TOF analysis and further characterized by whole-genome sequencing.

Results: This isolate was reliably identified as T. abortisuis and showed similar properties to type strain T. abortisuis DSM 19515^T, which was recovered from a sow following an abortion. The assembled genome of this isolate was 2 564 866 bp long with a GC content of 63.9%. A total of 30 virulence-related genes were determined, suggesting the pathogenic potential of this organism.

Conclusion and clinical relevance: This study details the first isolation of T. abortisuis from goats. The genotypic findings of this isolate will serve as a baseline description for any similar future studies.

本刊更多论文

首次从加拿大一只山羊体内分离出 Trueperella abortisuis 并对其进行全基因组测序。

目的：本研究报告了首次从山羊体内分离出一种流产特氏菌并进行了全基因组测序：本研究报告了首次从山羊体内分离出的流产特氏菌（Trueperella abortisuis），并对其进行了全基因组测序：动物和样本：从一只流产山羊的子宫中分离出流产嗜血杆菌：通过纯培养物表型和 MALDI-TOF 分析鉴定流产 T. abortisuis，并通过全基因组测序进一步鉴定其特征：结果：该分离株被可靠地鉴定为流产 T. abortisuis，并显示出与从流产后的母猪身上回收的类型菌株 T. abortisuis DSM 19515T 相似的特性。该分离株的基因组长度为 2 564 866 bp，GC 含量为 63.9%。共确定了 30 个毒力相关基因，表明该生物具有致病潜力：本研究详细介绍了首次从山羊体内分离出的流产T.该分离物的基因分型结果将作为今后任何类似研究的基线描述。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Canadian Veterinary Journal-revue Veterinaire Canadienne 农林科学-兽医学

CiteScore

1.20

自引率

10.00%

发文量

177

审稿时长

12-24 weeks

期刊介绍： The Canadian Veterinary Journal (CVJ) provides a forum for the discussion of all matters relevant to the veterinary profession. The mission of the Journal is to educate by informing readers of progress in clinical veterinary medicine, clinical veterinary research, and related fields of endeavor. The key objective of The CVJ is to promote the art and science of veterinary medicine and the betterment of animal health. A report suggesting that animals have been unnecessarily subjected to adverse, stressful, or harsh conditions or treatments will not be processed for publication. Experimental studies using animals will only be considered for publication if the studies have been approved by an institutional animal care committee, or equivalent, and the guidelines of the Canadian Council on Animal Care, or equivalent, have been followed by the author(s).