Lnc-216 regulates the miR-143-5p /MMP2 signaling axis aggravates retinal endothelial cell dysfunction.

Clinical hemorheology and microcirculation Pub Date : 2024-06-27 DOI:10.3233/CH-242163

Fang Wang, Zhangmei Guo, Guiqi Yang, Fan Yang, Qi Zhou, Hongbin Lv

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Abstract

Purpose: Diabetic retinopathy (DR) is a serious retinal vascular disease that affects many individuals in their prime working years. The present research aimed at whether and how LOC681216 (LNC-216) is involved in retinal vascular dysfunction under diabetic conditions.

Methods: Rat retinal microvascular endothelial cells (RRMECs) treated with high glucose (HG) were used for functional analysis. Gene expression analysis was conducted using the Clariom D Affymetrix platform. The wound healing, transwell, and vascular tube formation assays were used to identify the migration, invasion, and tube formation capability of RRMECs. The dual-luciferase reporter confirmed the binding interaction between miR-143-5p and LNC-216 or matrix metallopeptidase 2 (MMP2).

Results: Lnc-216 was upregulated in RRMECs treated with HG. Lnc-216 knockdown markedly suppressed the tube formation, cell migration, and wound healing of cultured RRMECs under HG conditions. Mechanistically, Lnc-216 acted as a miR-143-5p sponge to affect the biological activity of miR-143-5p, which led to increased expression of matrix metallopeptidase 2 (MMP2).

Conclusions: Lnc-216 attenuates diabetic retinal vascular dysfunction through the miR-143-5p/MMP2 axis, providing a potential therapeutic strategy for DR.

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Lnc-216 调节 miR-143-5p /MMP2 信号轴加重视网膜内皮细胞功能障碍。

目的：糖尿病视网膜病变（DR）是一种严重的视网膜血管疾病，影响着许多正值壮年的人。本研究旨在探讨 LOC681216（LNC-216）是否以及如何参与糖尿病条件下的视网膜血管功能障碍：方法：使用经高糖（HG）处理的大鼠视网膜微血管内皮细胞（RRMECs）进行功能分析。使用 Clariom D Affymetrix 平台进行基因表达分析。伤口愈合、transwell 和血管管形成试验用于鉴定 RRMECs 的迁移、侵袭和血管管形成能力。双荧光素酶报告物证实了 miR-143-5p 与 LNC-216 或基质金属肽酶 2（MMP2）之间的结合相互作用：结果：Lnc-216 在接受 HG 处理的 RRMECs 中上调。结果：Lnc-216 在经 HG 处理的 RRMECs 中上调，Lnc-216 基因敲除明显抑制了 HG 条件下培养的 RRMECs 的管形成、细胞迁移和伤口愈合。从机理上讲，Lnc-216作为miR-143-5p海绵影响了miR-143-5p的生物活性，从而导致基质金属肽酶2（MMP2）的表达增加：结论：Lnc-216通过miR-143-5p/MMP2轴减轻糖尿病视网膜血管功能障碍，为DR提供了一种潜在的治疗策略。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Clinical hemorheology and microcirculation

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