Mycobacterium tuberculosis Beijing in the State of Nuevo Leon, Mexico.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-06-27 DOI:10.1016/j.ram.2023.12.007

Nohemí Catana-Botello, Pola Becerril-Montes, Jorge Castro-Garza, Francisco González-Salazar, Horacio Almanza-Reyes, María De Los Ángeles Del Bosque-Moncayo, Alejandro Morales-Vargas, Víctor Manuel Velázquez-Moreno

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引用次数: 0

Abstract

Tuberculosis remains a serious threat to human health as an infectious disease in Mexico. Data about the genotypes of circulating Mycobacterium tuberculosis isolates (MTB) in the State of Nuevo Leon, Mexico are scarce. We aimed to determine the genotypes of circulating MTB belonging to the Beijing lineage recovered from patients in the State of Nuevo Leon, Mexico. A total of 406 MTB isolates from this state were genotyped using the spoligotyping method and 18-locus MIRU-VNTR. Lineage classification and MTB transmission analysis were performed. Based on the spoligotyping analysis, we found 24 strains belonging to the Beijing genotype that were characterized phylogenetically. The MIRUs showed greater discriminatory power than the standard RFLP-IS6110 method; therefore, the greatest allelic diversity among the Beijing strains was observed with MIRU10, MIRU31, MIRU39, MRU40, and MIRU 26. MVLA analysis showed a profile variation between Beijing and non-Beijing strains. The minimum spanning tree (MST) showed that 79% (19) of the strains are related. All Beijing strains exhibited the deletion of region TbD1, which is a characteristic of modern strains. The application of spoligotyping and MIRU-VNTR-18 methods together proved to be more sensitive, discriminatory, and rapid than the standard method for the epidemiological analysis of Mycobacterium Beijing isolates. This study is one of the first to describe the genomic diversity of M. Beijing in the State of Nuevo Leon, Mexico.

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墨西哥新莱昂州的北京结核分枝杆菌。

在墨西哥，结核病作为一种传染病仍然严重威胁着人类健康。有关墨西哥新莱昂州循环结核分枝杆菌（MTB）分离株基因型的数据很少。我们的目的是确定从墨西哥新莱昂州患者身上分离出的属于北京系的循环性 MTB 的基因型。我们使用spoligotyping方法和18-locus MIRU-VNTR对来自该州的406株MTB分离株进行了基因分型。进行了菌系分类和 MTB 传播分析。根据spoligotyping分析，我们发现了属于北京基因型的24株菌株，并对其进行了系统发育鉴定。与标准的 RFLP-IS6110 方法相比，MIRUs 显示出更强的分辨能力；因此，在北京菌株中，MIRU10、MIRU31、MIRU39、MRU40 和 MIRU26 的等位基因多样性最大。MVLA 分析表明，北京菌株与非北京菌株之间存在剖面差异。最小生成树（MST）显示，79% 的菌株（19 株）具有亲缘关系。所有北京菌株都表现出 TbD1 区的缺失，这是现代菌株的特征。事实证明，在对北京分枝杆菌分离株进行流行病学分析时，联合应用spoligotyping和MIRU-VNTR-18方法比标准方法更灵敏、更有鉴别力、更快速。这项研究是首次描述墨西哥新莱昂州北京分枝杆菌基因组多样性的研究之一。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.