How well do antimicrobial mouth rinses prevent dysbiosis in an in vitro periodontitis biofilm model?

IF 4.2 2区 医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE
Naiera Zayed, Rik Vertommen, Kenneth Simoens, Kristel Bernaerts, Nico Boon, Mrinal Gaurav Srivastava, Annabel Braem, Wannes Van Holm, Ana B. Castro, Wim Teughels
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引用次数: 0

Abstract

Background

Periodontal diseases are associated with dysbiosis in the oral microbial communities. Managing oral biofilms is therefore key for preventing these diseases. Management protocols often include over-the-counter antimicrobial mouth rinses, which lack data on their effects on the oral microbiome's ecology, bacterial composition, metabolic activity, and dysbiosis resilience. This study examined the efficacy of antimicrobial mouth rinses to halt dysbiosis in in vitro oral biofilms under periodontitis-simulating conditions.

Methods

Multispecies oral biofilms were grown on hydroxyapatite discs (HADs) and rinsed daily with one of six mouth rinses. Positive and negative controls were included. After three rinses, biofilms were analyzed with viability quantitative polymerase chain reaction and visualized using scanning electron microscopy. Supernatants of rinsed biofilms were used for metabolic activity analysis. In addition, human oral keratinocytes were exposed to rinsed biofilms to assess their inflammatory response. All outputs were analyzed for correlation using Spearman coefficient.

Results

Product-related changes were observed in the rinsed biofilms. Three of the six tested mouth rinses could significantly prevent dysbiosis with ≥30% reduction in pathobiont abundance relative to the control. These biofilms had lower metabolic activity, and the exposed human oral keratinocyte produced less interleukin-8. Interleukin-8 production correlated to both pathobiont quantity and the metabolic activity of the biofilms.

Conclusion

Some mouth rinses could support biofilm resilience and stop dysbiosis evolution in the biofilm model, with a clear product-related effect. Such mouth rinses can be considered for patients under maintenance/supportive periodontal therapy to prevent/delay disease recurrence. Others are more useful for different periodontal therapy stages.

Abstract Image

抗菌漱口水在体外牙周炎生物膜模型中预防菌群失调的效果如何?
背景:牙周疾病与口腔微生物群落的菌群失调有关。因此,管理口腔生物膜是预防这些疾病的关键。管理方案通常包括非处方抗菌漱口水,但缺乏有关这些漱口水对口腔微生物群生态、细菌组成、代谢活动和菌群失调恢复能力的影响的数据。本研究考察了抗菌漱口水在模拟牙周炎的体外口腔生物膜中阻止菌群失调的功效:多菌种口腔生物膜生长在羟基磷灰石盘(HAD)上,每天用六种漱口水中的一种漱口。其中包括阳性对照组和阴性对照组。漱口三次后,用活力定量聚合酶链反应分析生物膜,并用扫描电子显微镜观察。冲洗后的生物膜上清液用于代谢活性分析。此外,还将人类口腔角质细胞暴露于冲洗过的生物膜,以评估其炎症反应。所有结果均使用斯皮尔曼系数进行相关性分析:结果:在冲洗过的生物膜中观察到了与产品相关的变化。在六种测试的漱口水中,有三种能显著预防菌群失调,与对照组相比,病原体丰度降低了≥30%。这些生物膜的代谢活性较低,暴露的人类口腔角质细胞产生的白细胞介素-8较少。白细胞介素-8的产生与病原菌的数量和生物膜的代谢活性有关:结论:一些漱口水可以支持生物膜的恢复能力,并阻止生物膜模型中菌群失调的演变,其效果明显与产品有关。接受维护性/支持性牙周治疗的患者可以考虑使用这类漱口水,以预防/延缓疾病复发。其他漱口水则更适用于不同的牙周治疗阶段。
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来源期刊
Journal of periodontology
Journal of periodontology 医学-牙科与口腔外科
CiteScore
9.10
自引率
7.00%
发文量
290
审稿时长
3-8 weeks
期刊介绍: The Journal of Periodontology publishes articles relevant to the science and practice of periodontics and related areas.
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