Muscle-Protective Effect of Carnosine against Dexamethasone-Induced Muscle Atrophy in C2C12 Myotube.

IF 0.7 4区医学 Q4 NUTRITION & DIETETICS

Journal of nutritional science and vitaminology Pub Date : 2024-01-01 DOI:10.3177/jnsv.70.219

Md Mizanur Rahman, Anayt Ulla, Hiroki Moriwaki, Yusuke Yasukawa, Takayuki Uchida, Takeshi Nikawa

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Abstract

This study investigated the protective effect of carnosine and its components (L-histidine and β-alanine [HA]) against dexamethasone (Dex)-induced muscle atrophy in C2C12 myotubes. Myotubes were treated with Dex (10 μM) to induce muscle atrophy manifested by decreased myotube diameter, low myosin heavy chain content, and increased expression of muscle atrophy-associated ubiquitin ligases (Atrogin-1, MuRF-1, and Cbl-b). Carnosine (20 mM) treatment significantly improved the myotube diameter and MyHC protein expression level in Dex-treated C2C12 myotubes. It also downregulated the expression of Atrogin-1, MuRF-1, and Cbl-b and suppressed the expression of forkhead box O3 (FoxO3a) mediated by Dex. Furthermore, reactive oxygen species production was increased by Dex but was ameliorated by carnosine treatment. However, HA (20 mM), the component of carnosine, treatment was found ineffective in preventing Dex-induced protein damage. Therefore, based on above results it can be suggested that carnosine could be a potential therapeutic agent to prevent Dex-induced muscle atrophy compared to its components HA.

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肉碱对地塞米松诱导的 C2C12 肌管肌肉萎缩的保护作用

本研究探讨了肌肽及其成分（L-组氨酸和β-丙氨酸[HA]）对地塞米松（Dex）诱导的C2C12肌管肌肉萎缩的保护作用。用地塞米松（10 μM）处理肌管以诱导肌肉萎缩，表现为肌管直径减小、肌球蛋白重链含量降低以及肌肉萎缩相关泛素连接酶（Atrogin-1、MuRF-1和Cbl-b）表达增加。卡诺辛（20 mM）能显著改善经 Dex 处理的 C2C12 肌细胞管的直径和 MyHC 蛋白表达水平。它还下调了Atrogin-1、MuRF-1和Cbl-b的表达，并抑制了由Dex介导的叉头盒O3（FoxO3a）的表达。此外，活性氧的产生因 Dex 而增加，但在肌肽处理后有所改善。然而，肌肽的成分 HA（20 mM）对防止 Dex 诱导的蛋白质损伤无效。因此，根据上述结果，与肌肽的成分 HA 相比，肌肽可能是一种预防 Dex 诱导的肌肉萎缩的潜在治疗剂。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of nutritional science and vitaminology 医学-营养学

CiteScore

1.80

自引率

6.20%

发文量

审稿时长

6-12 weeks

期刊介绍： The Journal of Nutritional Science and Vitaminology is an international medium publishing in English of original work in all branches of nutritional science, food science and vitaminology from any country. Manuscripts submitted for publication should be as concise as possible and must be based on the results of original research or of original interpretation of existing knowledge not previously published. Although data may have been reported, in part, in preliminary or abstract form, a full report of such research is unacceptable if it has been or will be submitted for consideration by another journal.