Alginate-gelatine hydrogel microspheres protect NK cell proliferation and cytotoxicity under hypoxic conditions.

IF 3 4区医学 Q2 CHEMISTRY, APPLIED

Journal of microencapsulation Pub Date : 2024-08-01 Epub Date: 2024-06-30 DOI:10.1080/02652048.2024.2362170

Jiyoung Cheon, Myeongkwan Song, Soonjo Kwon

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引用次数: 0

Abstract

Aims: This study aimed to encapsulate natural killer (NK) cells in a hydrogel to sustain their function within the hypoxic tumour microenvironments.

Methods: An alginate-gelatine hydrogel was generated via electrospray technology. Hydrogel biocompatibility was assessed through cell counting kit-8 and Live/Dead assays to ascertain cell. Moreover, we analysed lactate dehydrogenase assays to evaluate the cytotoxicity against tumours and utilised RT-qPCR to analyse cytokine gene level.

Results: Alginate and gelatine formed hydrogels with diameters ranging from 489.2 ± 23.0 μm, and the encapsulation efficiency was 34.07 ± 1.76%. Encapsulated NK cells exhibited robust proliferation and tumour-killing capabilities under normoxia and hypoxia. Furthermore, encapsulation provided a protective shield against cell viability under hypoxia. Importantly, tumour-killing cytotoxicity through cytokines upregulation such as granzyme B and interferon-gamma was preserved under hypoxia.

Conclusion: The encapsulation of NK cells not only safeguards their viability but also reinforces anticancer capacity, countering the inhibition of activation induced by hypoxia.

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藻酸盐-明胶水凝胶微球可在缺氧条件下保护 NK 细胞增殖和细胞毒性。

目的：本研究旨在将自然杀伤（NK）细胞包裹在水凝胶中，以维持其在缺氧肿瘤微环境中的功能：方法：通过电喷雾技术生成藻酸盐-明胶水凝胶。水凝胶的生物相容性通过细胞计数试剂盒-8 和活/死试验进行评估，以确定细胞。此外，我们还分析了乳酸脱氢酶试验，以评估对肿瘤的细胞毒性，并利用 RT-qPCR 分析细胞因子基因水平：海藻酸盐和明胶形成的水凝胶直径为 489.2 ± 23.0 μm，封装效率为 34.07 ± 1.76%。封装后的 NK 细胞在常氧和缺氧条件下均表现出强大的增殖和杀伤肿瘤能力。此外，在缺氧条件下，封装还能保护细胞存活。重要的是，在缺氧条件下，通过细胞因子（如颗粒酶 B 和干扰素-γ）上调产生的肿瘤杀伤细胞毒性得以保留：结论：对 NK 细胞进行封装不仅能保障其生存能力，还能增强抗癌能力，抵消缺氧对激活的抑制作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of microencapsulation 工程技术-工程：化工

CiteScore

6.30

自引率

2.60%

发文量

审稿时长

3 months

期刊介绍： The Journal of Microencapsulation is a well-established, peer-reviewed journal dedicated to the publication of original research findings related to the preparation, properties and uses of individually encapsulated novel small particles, as well as significant improvements to tried-and-tested techniques relevant to micro and nano particles and their use in a wide variety of industrial, engineering, pharmaceutical, biotechnology and research applications. Its scope extends beyond conventional microcapsules to all other small particulate systems such as self assembling structures that involve preparative manipulation. The journal covers: Chemistry of encapsulation materials Physics of release through the capsule wall and/or desorption from carrier Techniques of preparation, content and storage Many uses to which microcapsules are put.