Helicobacter pylori vacA allelic combination, dupA, cagE and cagA genotypes and their associations with gastric diseases among Moroccan population.

IF 1.3 4区 医学 Q4 INFECTIOUS DISEASES
Souad Oirdi Zahir, Mounia El Khadir, Samia Alaoui Boukhris, Dafr-Allah Benajah, Sidi Adil Ibrahimi, Laila Chbani, Mohamed El Abkari, Bahia Bennani
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Abstract

The combination of the four regions of vacA with cagA, cagE, dupA genes and cagA-EPIYA motifs, was studied to find the most likely combination that can be used as a disease determinant marker in Moroccan population. A total of 838 H. pylori positive obtained from consenting patients, that were previously analyzed by PCR to characterize vacA-s -m, -i regions, cagE status and cagA 3' region polymorphism, were used to characterize vacA-d region and to determine dupA gene status. The analysis shows the predominance of the less virulent combination (vacA(s2m2i2d2)dupA(-)cagE(-)cagA(-)), and shows that the risk of gastric cancer is 13.33 fold higher (1.06-166.37)) in patients infected by strains harboring vacA(s1m1i1d1)dupA(-)cagE(+)cagA(2EPIYA-C) compared to patients with gastritis without lesions and infected by H.pylori strains harboring vacA(s2m2i2d2)dupA(-)cagE(-)cagA(-). The infection with strains harboring vacA(s1m1i1d1)dupA(+)cagE(+)cagA(1EPIYAC) genotype combination represents a risk factor for gastric ulcer and duodenal ulcer (the Odds Ratio (95% CI) were 16 (1.09-234.24) and 6.54 (1.60-26.69) respectively) compared to patients with gastritis without lesions. These results suggest that the combination of the active form of vacA genotypes, dupA gene status and the number of EPIYA-C motif may be considered helpful markers to discriminate between several gastric diseases.

幽门螺杆菌 vacA 等位基因组合、dupA、cagE 和 cagA 基因型及其与摩洛哥人胃病的关系。
研究人员对 vacA 的四个区域与 cagA、cagE、dupA 基因和 cagA-EPIYA motifs 的组合进行了研究,以找到最有可能用作摩洛哥人群疾病决定性标记的组合。从征得同意的患者身上共获得了 838 例幽门螺杆菌阳性病例,这些病例之前已通过 PCR 分析确定了 vacA-s -m、-i 区域、cagE 状态和 cagA 3' 区域多态性的特征,现在又用来确定 vacA-d 区域的特征和 dupA 基因的状态。分析结果表明,毒力较弱的组合(vacA(s2m2i2d2)dupA(-)cagE(-)cagA(-))占主导地位,并显示胃癌风险在感染了vacA-d区的患者中高出13.33倍(1.06-166.37)),而感染了携带 vacA(s1m1i1d1)dupA(-)cagE(+)cagA(2EPIYA-C) 的幽门螺杆菌菌株的无病变胃炎患者要比感染了携带 vacA(s2m2i2d2)dupA(-)cagE(-)cagA(-) 的幽门螺杆菌菌株的患者高出 13.33 倍。与无病变的胃炎患者相比,感染携带 vacA(s1m1i1d1)dupA(+)cagE(+)cagA(1EPIYAC) 基因型组合的菌株是胃溃疡和十二指肠溃疡的危险因素(Odds Ratio (95% CI) 分别为 16 (1.09-234.24) 和 6.54 (1.60-26.69))。这些结果表明,vacA 基因型的活性形式、dupA 基因状态和 EPIYA-C 矩阵的数量可被认为是区分多种胃病的有用标记物。
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来源期刊
CiteScore
4.50
自引率
4.50%
发文量
172
审稿时长
2 months
期刊介绍: Japanese Journal of Infectious Diseases (JJID), an official bimonthly publication of National Institute of Infectious Diseases, Japan, publishes papers dealing with basic research on infectious diseases relevant to humans in the fields of bacteriology, virology, mycology, parasitology, medical entomology, vaccinology, and toxinology. Pathology, immunology, biochemistry, and blood safety related to microbial pathogens are among the fields covered. Sections include: original papers, short communications, epidemiological reports, methods, laboratory and epidemiology communications, letters to the editor, and reviews.
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