Combination of Autophagy and Stem Cell Enhancing Properties of Natural Product Extracts in Human Dermal Papilla Stem Cells.

IF 1.8 4区医学 Q3 MEDICINE, RESEARCH & EXPERIMENTAL

In vivo Pub Date : 2024-07-01 DOI:10.21873/invivo.13627

Zin Zin Ei, Supalerk Kowinthanaphat, Pilaiwanwadee Hutamekalin, Verisa Chowjarean, Pithi Chanvorachote

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引用次数: 0

Abstract

Background/aim: Dermal papilla (DP) stem cells are known for their remarkable regenerative capacity, making them a valuable model for assessing the effects of natural products on cellular processes, including stemness, and autophagy.

Materials and methods: Autophagy and stemness characteristics were assessed using real-time RT-PCR to analyze mRNA levels, along with immunofluorescence and western blot techniques for protein level evaluation.

Results: Butterfly Pea, Emblica Fruits, Kaffir Lime, and Thunbergia Laurifolia extracts induced autophagy in DP cells. Kaffir Lime-treated cells exhibited increase in the OCT4, NANOG, and SOX2 mRNA (6-, 5, and 5.5-fold, respectively), and protein levels (4-, 3-, and 1.5-fold, respectively). All extracts activated the survival protein kinase B (Akt) in DP cells.

Conclusion: Natural products are a promising source for promoting hair growth by rejuvenating hair stem cells.

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天然产品提取物在人类真皮乳头干细胞中的自噬和干细胞增强特性的结合。

背景/目的：众所周知，真皮乳头（DP）干细胞具有显著的再生能力，因此是评估天然产品对细胞过程（包括干性和自噬）影响的宝贵模型：采用实时 RT-PCR 分析 mRNA 水平，以及免疫荧光和 Western 印迹技术评估蛋白质水平，从而评估自噬和干性特征：结果：蝴蝶豌豆、恩比西亚果、卡菲尔石灰和月桂叶提取物诱导了DP细胞的自噬。卡菲尔石灰处理过的细胞显示出 OCT4、NANOG 和 SOX2 mRNA（分别为 6、5 和 5.5 倍）和蛋白质水平（分别为 4、3 和 1.5 倍）的增加。所有提取物都能激活 DP 细胞中的存活蛋白激酶 B（Akt）：结论：天然产品是通过使毛发干细胞恢复活力来促进毛发生长的一个很有前景的来源。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

In vivo 医学-医学：研究与实验

CiteScore

4.20

自引率

4.30%

发文量

330

审稿时长

3-8 weeks

期刊介绍： IN VIVO is an international peer-reviewed journal designed to bring together original high quality works and reviews on experimental and clinical biomedical research within the frames of physiology, pathology and disease management. The topics of IN VIVO include: 1. Experimental development and application of new diagnostic and therapeutic procedures; 2. Pharmacological and toxicological evaluation of new drugs, drug combinations and drug delivery systems; 3. Clinical trials; 4. Development and characterization of models of biomedical research; 5. Cancer diagnosis and treatment; 6. Immunotherapy and vaccines; 7. Radiotherapy, Imaging; 8. Tissue engineering, Regenerative medicine; 9. Carcinogenesis.