Effect of co-administration of gallic acid and quercetin or gallic acid and rutin on impaired spermatogenesis and oxidative damage in a busulfan-treated rat model.
Ogechukwu E Ezim, Chisom E Nebeolisa, Ifeoma G Emeagwali-John, Victoria C Obinna, Sunny O Abarikwu
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引用次数: 0
Abstract
Gallic acid (GAL), rutin (RUT), and quercetin (QUE) are common antioxidant agents in fruits and vegetables with intriguing pharmacological effects. In the present study, we compared the therapeutic outcomes of GAL + QUE in comparison with GAL + RUT co-treatment in a busulfan (BUS) model of testicular injury in Wistar rats. BUS (4 mg kg-1 body weight (b.w) was injected intraperitoneally daily for 4 days. GAL + RUT or GAL + QUE (20 mg kg-1 b. w) was delivered by oral gavage for 52 days. Examination of the testes of BUS-treated rats both biochemically and under light microscopy revealed an increased level of lipid peroxidation, DNA fragmentation, glutathione-S-transferase, lactate dehydrogenase, gamma-glutamyl transpeptidase, alkaline phosphatase and acid phosphatase with a concomitant decrease in the level of antioxidants: glutathione, ascorbic acid, superoxide dismutase, catalase, glutathione peroxidase and glutathione reductase activities, suggesting testicular injury. Tissue sections confirmed the testicular injury-induced by BUS, including diminished spermatogenesis score index, tubular diameter, gonado-somatic index, testis weight, epithelia thickness and higher percentage of aberrant tubules. GAL + QUE co-administration had better recovery effects than GAL + RUT on the biochemical markers and protected against BUS-induced testicular damage. GAL + QUE treatment regimen has better capacity to maintain the antioxidant capacity of the testes and is more potent at reducing BUS-induced oxidative damage compared to GAL + RUT.
没食子酸（GAL）、芦丁（RUT）和槲皮素（QUE）是水果和蔬菜中常见的抗氧化剂，其药理作用引人入胜。在本研究中，我们比较了 GAL + QUE 与 GAL + RUT 联合治疗 Wistar 大鼠睾丸损伤模型的疗效。每天腹腔注射4毫克/千克体重（b.w）的BUS，连续4天。口服 GAL + RUT 或 GAL + QUE（20 毫克/千克体重），连续 52 天。对 BUS 处理过的大鼠睾丸进行生化和光学显微镜检查后发现，脂质过氧化、DNA 断裂、谷胱甘肽-S-转移酶、乳酸脱氢酶、γ-谷氨酰转肽酶、碱性磷酸酶和酸性磷酸酶的水平均有所上升，抗氧化剂的水平也随之下降：谷胱甘肽、抗坏血酸、超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶和谷胱甘肽还原酶等抗氧化剂的活性也随之降低，这表明睾丸受到了损伤。组织切片证实了 BUS 引起的睾丸损伤，包括精子发生评分指数、小管直径、性腺指数、睾丸重量、上皮厚度和畸形小管百分比增加。与 GAL + RUT 相比，GAL + QUE 联合用药对生化指标有更好的恢复效果，并能防止 BUS 引起的睾丸损伤。与 GAL + RUT 相比，GAL + QUE 治疗方案能更好地维持睾丸的抗氧化能力，并能更有效地减少 BUS 引起的氧化损伤。
期刊介绍：
Drug and Chemical Toxicology publishes full-length research papers, review articles and short communications that encompass a broad spectrum of toxicological data surrounding risk assessment and harmful exposure. Manuscripts are considered according to their relevance to the journal.
Topics include both descriptive and mechanics research that illustrates the risk assessment implications of exposure to toxic agents. Examples of suitable topics include toxicological studies, which are structural examinations on the effects of dose, metabolism, and statistical or mechanism-based approaches to risk assessment. New findings and methods, along with safety evaluations, are also acceptable. Special issues may be reserved to publish symposium summaries, reviews in toxicology, and overviews of the practical interpretation and application of toxicological data.
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