Formin Binding Protein 1 (FNBP1) regulates non-canonical Wnt signaling and vertebrate gastrulation (12 words)

IF 2.5 3区生物学 Q2 DEVELOPMENTAL BIOLOGY

Developmental biology Pub Date : 2024-06-28 DOI:10.1016/j.ydbio.2024.06.019

Courtney Zeni, Yuko Komiya, Raymond Habas

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Abstract

The Formin protein Daam1 is required for Wnt-induced cytoskeletal changes during gastrulation, though how it accomplishes this remains unresolved. Here we report the characterization of Formin Binding Protein 1 (FNBP1) as a binding partner of Daam1. The interaction of Daam1 with FNBP1 and its domains required for this interaction were delineated. Immunofluorescence studies showed FNBP1 co-localizes with Daam1, and is an integral component of the actin cytoskeletal complex that is responsive to Wnt stimulation. Specifically, FNBP1 can induce intracellular tubule-like structures and localize to focal adhesions suggesting a role for FNBP1 in cell migration. Functional FNBP1 studies in Xenopus embryos uncover a critical role for FNBP1 in regulating vertebrate gastrulation. Additionally, suboptimal doses of Daam1 and FNBP1 synergize to produce severe gastrulation defects, indicating FNBP1 and Daam1 may function within the same signaling pathway. These results together show FNBP1 is an integral component of Daam1-regulated non-canonical Wnt signaling required for vertebrate gastrulation.

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福尔马林结合蛋白 1（FNBP1）调控非典型 Wnt 信号传导和脊椎动物的胃形成（12 个字）。

在胃形成过程中，Wnt 诱导的细胞骨架变化需要福尔马林蛋白 Daam1，但它是如何实现这一目标的仍未解决。在这里，我们报告了作为 Daam1 结合伙伴的福尔马林结合蛋白 1（FNBP1）的特征。我们描述了 Daam1 与 FNBP1 的相互作用及其相互作用所需的结构域。免疫荧光研究显示，FNBP1与Daam1共定位，是肌动蛋白细胞骨架复合体的一个组成部分，对Wnt刺激有反应。特别是，FNBP1 能诱导细胞内的管状结构，并定位到病灶粘连处，这表明 FNBP1 在细胞迁移中发挥作用。在爪蟾胚胎中进行的 FNBP1 功能研究发现，FNBP1 在调节脊椎动物的胃形成过程中起着关键作用。此外，次优剂量的 Daam1 和 FNBP1 会协同产生严重的胃形成缺陷，这表明 FNBP1 和 Daam1 可能在同一信号通路中发挥作用。这些结果共同表明，FNBP1是脊椎动物胃形成所需的Daam1调控的非经典Wnt信号的一个组成部分。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Developmental biology 生物-发育生物学

CiteScore

5.30

自引率

3.70%

发文量

182

审稿时长

1.5 months

期刊介绍： Developmental Biology (DB) publishes original research on mechanisms of development, differentiation, and growth in animals and plants at the molecular, cellular, genetic and evolutionary levels. Areas of particular emphasis include transcriptional control mechanisms, embryonic patterning, cell-cell interactions, growth factors and signal transduction, and regulatory hierarchies in developing plants and animals.