METTL14 mediates nerve growth factor-stimulated testosterone synthesis in porcine theca cells†.

IF 4.3 3区 材料科学 Q1 ENGINEERING, ELECTRICAL & ELECTRONIC
Yuxin Luo, Yun Zhao, Boqi Zhang, Tong Chen, Xue Chen, Caomeihui Shen, Guitian He, Maosheng Cao, Lu Chen, Yueying Wang, Nan Wang, Jinxin Zong, Xu Zhou, Chunjin Li
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引用次数: 0

Abstract

Ovarian theca cells produce testosterone, which acts as a vital precursor substance for synthesizing estrogens during follicular development. Nerve growth factor (NGF) has been shown to participate in reproductive physiology, specifically to follicular development and ovulation. There is currently no available data on the impact of NGF on testosterone synthesis in porcine theca cells. Furthermore, m6A modification is the most common internal modification in eukaryotic mRNAs that are closely associated with female gametogenesis, follicle development, ovulation, and other related processes. It is also uncertain whether the three main enzymes associated with m6A, such as Writers, Erasers, and Readers, play a role in this process. The present study, with an in vitro culture model, investigated the effect of NGF on testosterone synthesis in porcine theca cells and the role of Writers-METTL14 in this process. It was found that NGF activates the PI3K/AKT signaling pathway through METTL14, which regulates testosterone synthesis in porcine theca cells. This study will help to further elucidate the mechanisms by which NGF regulates follicular development and provide new therapeutic targets for ovary-related diseases in female animals. Summary Sentence  The present study investigated the effect of NGF on testosterone synthesis in porcine theca cells. It was found that NGF activates the PI3K/AKT signaling pathway through METTL14, which regulates testosterone synthesis in porcine theca cells.

METTL14介导猪绒毛膜细胞中神经生长因子刺激的睾酮合成。
卵巢癌细胞产生睾酮,睾酮是卵泡发育过程中合成雌激素的重要前体物质。神经生长因子(NGF)已被证明参与生殖生理,特别是卵泡发育和排卵。目前还没有关于 NGF 对猪癌细胞中睾酮合成的影响的数据。此外,m6A 修饰是真核生物 mRNA 中最常见的内部修饰,与女性配子发生、卵泡发育、排卵和其他相关过程密切相关。目前还不确定与 m6A 相关的三种主要酶,如写入器、擦除器和读取器是否在这一过程中发挥作用。本研究采用体外培养模型,研究了 NGF 对猪绒毛膜细胞睾酮合成的影响以及 Writers-METTL14 在这一过程中的作用。研究发现,NGF通过METTL14激活PI3K/AKT信号通路,从而调节猪癌细胞的睾酮合成。这项研究将有助于进一步阐明NGF调节卵泡发育的机制,并为雌性动物卵巢相关疾病提供新的治疗靶点。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
7.20
自引率
4.30%
发文量
567
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