A novel p.127Val>Ile single nucleotide polymorphism in the MTNR1A gene and its relation to litter size in Thin-tailed Indonesian ewes.

IF 2.4 2区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Animal Bioscience Pub Date : 2025-02-01 Epub Date: 2024-06-25 DOI:10.5713/ab.24.0187

Mutasem Abuzahra, Mohammed Baqur S Al-Shuhaib, Dwi Wijayanti, Mustofa Helmi Effendi, Imam Mustofa, Ikechukwu Benjamin Moses

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Abstract

Objective: The primary objective was to identify and characterize the single nucleotide polymorphisms (SNPs) within the MTNR1A gene sequence in Thin-tailed Indonesian ewes to assess the possible association of MTNR1A gene polymorphism with litter size trait.

Methods: Forty-seven Thin-tailed Indonesian sheep were selected for the study. Genotyping involved collecting blood samples, and sequencing exon 2 of the MTNR1A gene.

Results: The study identified 19 novel SNPs, with 10 being non-synonymous variations, in the MTNR1A gene of Thin-tailed Indonesian ewes. One non-synonymous SNP (rs1087815963) showed a significant association with litter size, with the GC genotype exhibiting a higher average litter size than the GG genotype. The deleterious impact of p.Val127Ile SNP was predicted by various in silico tools that predicted a highly damaging effect of p.Val127Ile SNP on the structure, function, and stability of MTNR1A. Docking reactions showed a critical involvement of this locus with the binding with melatonin.

Conclusion: In conclusion, the results of our study suggest that rs1087815963 has a remarkable negative impact on the MTNR1A with a putative alteration in the binding with melatonin. Therefore, the implementation of the novel p.Val127Ile could be a useful marker in marker-assisted selection.

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薄尾印尼母羊 MTNR1A 基因中一个新的 p.127Val>Ile SNP 及其与产仔数的关系。

研究目的主要目的是鉴定印尼细尾母羊 MTNR1A 基因序列中的单核苷酸多态性（SNPs）并确定其特征，以评估 MTNR1A 基因多态性与产仔数性状的可能关联：研究选取了47只印尼细尾绵羊。基因分型包括采集血液样本和 MTNR1A 基因第 2 外显子测序：研究在印尼细尾羊的 MTNR1A 基因中发现了 19 个新 SNPs，其中 10 个为非同义变异。其中一个非同义 SNP（rs1087815963）与产仔数有显著关联，GC 基因型的母羊平均产仔数高于 GG 基因型的母羊。通过各种硅学工具预测了p.Val127Ile SNP的有害影响，这些工具预测p.Val127Ile SNP对MTNR1A的结构、功能和稳定性具有高度破坏性影响。对接反应显示，该基因位点与褪黑激素的结合有重要关系：总之，我们的研究结果表明，rs1087815963 对 MTNR1A 有显著的负面影响，可能会改变其与褪黑激素的结合。因此，可以说新型 p.Val127Ile 可作为标记辅助选择的有用标记。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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