Ultrasound-assisted extraction of phenolics from Sargassum carpophyllum – A kinetics study

Abstract

The species of the brown macroalgal genus Sargassum are distributed globally and contain many bioactive compounds. In this study, ultrasound-assisted extraction (UAE) was applied to obtain phenolic compounds with strong antioxidant activity from Sargassum carpophyllum collected along the coastline of Weizhou Island in the South China Sea. The influence of different variables such as the solid–liquid ratio (1:5–1:30 g · mL⁻¹), ultrasonic power (160–280 W), duty circle ratio (DCR, 1/3–1/1), and ethanol concentration (30% to ~90%) were studied using a single factor design. The extraction kinetics were investigated using the Peleg model and second-order kinetics model, and the second-order model described the extraction procedure better than the Peleg model. Total phenol content (TPC) values of 3.316, 2.964, 2.741, and 3.665 mg phloroglucinol (PHG) · g⁻¹ algae were achieved at a higher solid–liquid ratio (1:30 g · mL⁻¹), higher ultrasonic power (280 W), a higher DCR (1/1), and a moderate ethanol concentration (50%), respectively. However, a slightly different result was observed in the extract obtained, with total phenol contents (TPC_extract) of 52.99, 65.00, 46.22, and 55.10 mg PHG · g⁻¹ extract and DPPH radical scavenging activity (IC50) of 0.096, 0.066, 0.131, and 0.136 mg extract · mL⁻¹ observed at 50% ethanol, 1:5 g m· mL⁻¹, 2/3 DCR, and 200 W respectively. All variables studied influenced the extraction kinetics by altering the extraction rate and the TPC at equilibrium. As for the bioactivities in the extract, a larger solid–liquid ratio and greater ultrasonic power may not contribute because of their ability to extract non-phenolic components simultaneously, leading to reduced overall bioactivities. The results of the present study provide essential information for future UAE process design and optimization for extracting phenolics from S. carpophyllum through mathematical modeling and could be regarded as important reference for obtaining value-added products from other macroalgae species.