Zn solubilizing bacteria (ZSB) mitigate toxicity of silver and Titanium dioxide nanoparticles in Mung bean by increasing photosynthetic pigment content.

IF 0.8 4区 生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Mahvash Haroon, Shams Tabrez Khan, Abdul Malik
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引用次数: 0

Abstract

Zn-deficiency, a global health challenge affects one-third of the world population. Zn-biofertilizer offer an efficient and cost-effective remedy. As Zn-biofertilizer can improve plant growth and grain's Zn-content ensuring improved dietary Zn-supply. This study sought to understand how silver and TiO2 nanoparticles in the rhizosphere affect the activity of Zn-solubilization bacteria (ZSB) and plant growth. Two ZSB strains Bacillus sp. D-7 and Pseudomonas sp. D-117 with excellent Zn-solubilization efficiency of 254 and 260%, respectively were isolated and characterized using polyphasic characterization including 16S rRNA gene sequencing to formulate an effective Zn-biofertilizer. The plant growth promoting activity of this biofertilizer in Mung bean was checked in the presence and absence of various doses of TiO2 and Ag-NPs and was compared with plant grown without biofertilizer. The change in rate of seed germination, vegetative growth (shoot and root length, fresh and dry weight), photosynthetic pigment and Zn-content was checked. Lower doses of nanomaterials (50 and 100 mg kg⁻¹ soil) slightly promoted the plant growth compared to control. While, higher doses (200 and 400 mg kg⁻¹ soil) inhibited the growth. A maximum decrease of shoot length, root length, fresh-weight, and dry-weight of 57.1, 53.9, 53.1, and 10.4% respectively was observed with 400 mg kg⁻¹ of Ag-NPs. However, in the presence of ZSB, the decrease at the same Ag-NP concentration was 41.6, 31.5, 27.4, and 6.6, respectively. These results strongly suggest that Zn-solubilizing bacteria improve resilience to nanoparticles toxicity and helps in Zn fortification in Mung bean even under nanomaterial stress.

锌溶解菌(ZSB)通过增加光合色素含量来减轻纳米银和二氧化钛对绿豆的毒性。
缺锌是一项全球性的健康挑战,影响着全球三分之一的人口。锌生物肥料是一种高效、经济的补救措施。锌生物肥料可以改善植物生长和谷物的锌含量,确保改善膳食锌供应。本研究试图了解根圈中的银和二氧化钛纳米粒子如何影响锌溶解菌(ZSB)的活性和植物生长。通过多相表征(包括 16S rRNA 基因测序),分离并鉴定了两株 ZSB 菌株芽孢杆菌 D-7 和假单胞菌 D-117,它们对锌的溶解效率分别为 254% 和 260%。在有和没有不同剂量的 TiO2 和 Ag-NPs 的情况下,检测了这种生物肥料对绿豆植物生长的促进作用,并与没有使用生物肥料的植物进行了比较。检测了种子发芽率、无性生长(芽和根的长度、鲜重和干重)、光合色素和锌含量的变化。与对照组相比,较低剂量的纳米材料(50 和 100 毫克/千克-¹ 土壤)略微促进了植物的生长。而较高剂量(200 和 400 毫克/千克/¹土壤)则会抑制生长。在使用 400 毫克/千克的 Ag-NPs 时,观察到芽长、根长、鲜重和干重的最大降幅分别为 57.1%、53.9%、53.1% 和 10.4%。然而,在有 ZSB 存在的情况下,相同 Ag-NP 浓度下的降幅分别为 41.6%、31.5%、27.4% 和 6.6%。这些结果有力地表明,锌溶解菌提高了对纳米粒子毒性的抵抗力,即使在纳米材料压力下也有助于绿豆的锌强化。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of General and Applied Microbiology
Journal of General and Applied Microbiology 生物-生物工程与应用微生物
CiteScore
2.40
自引率
0.00%
发文量
42
审稿时长
6-12 weeks
期刊介绍: JGAM is going to publish scientific reports containing novel and significant microbiological findings, which are mainly devoted to the following categories: Antibiotics and Secondary Metabolites; Biotechnology and Metabolic Engineering; Developmental Microbiology; Environmental Microbiology and Bioremediation; Enzymology; Eukaryotic Microbiology; Evolution and Phylogenetics; Genome Integrity and Plasticity; Microalgae and Photosynthesis; Microbiology for Food; Molecular Genetics; Physiology and Cell Surface; Synthetic and Systems Microbiology.
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