Monitoring Influenza C and D Viruses in Patients With Respiratory Diseases in Japan, January 2018 to March 2023

IF 4.3 4区医学 Q1 INFECTIOUS DISEASES

Influenza and Other Respiratory Viruses Pub Date : 2024-06-24 DOI:10.1111/irv.13345

Kohei Shimizu, Chiharu Kawakami, Yoko Matsuzaki, Seiichiro Fujisaki, Shiho Nagata, Hiroko Morita, Kayo Watanabe, Hideka Miura, Tomoko Momoki, Miwako Saikusa, Hiroki Ozawa, Makoto Kumazaki, Shuzo Usuku, Nobuko Tanaka, Ryuichi Senda, Ichiro Okubo, Shinji Watanabe, Hideki Hasegawa, Yoshihiro Kawaoka, Emi Takashita

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引用次数: 0

Abstract

Background

Influenza viruses can cause zoonotic infections that pose public health risks. Surveillance of influenza A and B viruses is conducted globally; however, information on influenza C and D viruses is limited. Longitudinal monitoring of influenza C virus in humans has been conducted in several countries, but there has been no long-term monitoring of influenza D virus in humans. The public health risks associated with the influenza D virus therefore remain unknown.

Methods

We established a duplex real-time RT-PCR to detect influenza C and D viruses and analyzed respiratory specimens collected from 2144 patients in Japan with respiratory diseases between January 2018 and March 2023. We isolated viruses and conducted hemagglutination inhibition tests to examine antigenicity and focus reduction assays to determine susceptibility to the cap-dependent endonuclease inhibitor baloxavir marboxil.

Results

We detected three influenza C viruses belonging to the C/Kanagawa- or C/Sao Paulo-lineages, which recently circulated globally. None of the specimens was positive for the influenza D virus. The C/Yokohama/1/2022 strain, isolated from the specimen with the highest viral RNA load and belonging to the C/Kanagawa-lineage, showed similar antigenicity to the reference C/Kanagawa-lineage strain and was susceptible to baloxavir.

Conclusions

Our duplex real-time RT-PCR is useful for the simultaneous detection of influenza C and D viruses from the same specimen. Adding the influenza D virus to the monitoring of the influenza C virus would help in assessing the public health risks posed by this virus.

查看原文本刊更多论文

2018年1月至2023年3月日本呼吸道疾病患者中的丙型和丁型流感病毒监测。

背景：流感病毒可引起人畜共患病，对公共健康构成威胁。全球都在对甲型和乙型流感病毒进行监测，但有关丙型和丁型流感病毒的信息却很有限。一些国家已对人类感染的丙型流感病毒进行了纵向监测，但尚未对人类感染的丁型流感病毒进行长期监测。因此，与 D 型流感病毒相关的公共卫生风险仍然未知：我们建立了一种检测丙型和丁型流感病毒的双重实时 RT-PCR，并分析了 2018 年 1 月至 2023 年 3 月期间从日本 2144 名呼吸道疾病患者身上采集的呼吸道标本。我们分离了病毒，并进行了血凝抑制试验以检测抗原性，还进行了聚焦还原试验以确定对帽依赖性内切酶抑制剂巴洛沙韦 marboxil 的敏感性：结果：我们检测到三种丙型流感病毒，它们属于最近在全球流行的丙型/神奈川系或丙型/圣保罗系。没有一个样本对丁型流感病毒呈阳性反应。从病毒 RNA 负荷最高的标本中分离出的 C/ 横滨/1/2022 株属于 C/ 神奈川系，其抗原性与参考的 C/ 神奈川系毒株相似，并且对巴洛沙韦敏感：结论：我们的双重实时 RT-PCR 技术可用于同时检测同一样本中的丙型和丁型流感病毒。在监测丙型流感病毒的同时加入丁型流感病毒，将有助于评估该病毒对公众健康造成的风险。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Influenza and Other Respiratory Viruses 医学-病毒学

CiteScore

7.20

自引率

4.50%

发文量

120

审稿时长

6-12 weeks

期刊介绍： Influenza and Other Respiratory Viruses is the official journal of the International Society of Influenza and Other Respiratory Virus Diseases - an independent scientific professional society - dedicated to promoting the prevention, detection, treatment, and control of influenza and other respiratory virus diseases. Influenza and Other Respiratory Viruses is an Open Access journal. Copyright on any research article published by Influenza and Other Respiratory Viruses is retained by the author(s). Authors grant Wiley a license to publish the article and identify itself as the original publisher. Authors also grant any third party the right to use the article freely as long as its integrity is maintained and its original authors, citation details and publisher are identified.