IL-10 indirectly modulates functional activity of CD4+CD28null T-lymphocytes through LFA-3 and HLA class II inhibition

IF 4.9 3区 医学 Q2 IMMUNOLOGY
Immunology Pub Date : 2024-06-23 DOI:10.1111/imm.13824
Alejandra García-Torre, Eva Bueno-García, Marco A. Moro-García, Rocío López-Martínez, Beatriz Rioseras, Beatriz Díaz-Molina, José Luis Lambert, Rebeca Alonso-Arias
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Abstract

Expansion of CD4+CD28null T-lymphocytes is common in chronic heart failure (CHF) patients. Its ability to produce high levels of proinflammatory cytokines is probably the key role of these cells in CHF. IL-10 is a candidate for limiting CD4+CD28null T-lymphocyte responses, whereas tumour necrosis factor (TNF) is the cytokine most closely involved in the loss of CD28 expression. Serum levels of TNF and IL-10 were measured in 65 CHF patients (mean age, 65.2 ± 13.84 years). Patients with an IL-10/TNF ratio ≥1 had significantly lower levels of CD4+CD28null T-lymphocytes than those with a ratio <1. In vitro, IL-10 reduced the frequency of proliferative CD4+CD28null T-lymphocytes stimulated with anti-CD3. Pre-treatment with IL-10 before anti-CD3 stimulation was required for the cytokine to inhibit TNF production by CD4+CD28null T-lymphocytes. In addition to the previously described effect of IL-10 on HLA-DR and ICAM-1 expression, LFA-3 protein and mRNA levels were reduced in the presence of the cytokine in monocytes. IL-10 inhibition on CD4+CD28null T-lymphocytes may be mediated by a reduction in HLA class II and LFA-3 expression because blocking interactions with these costimulators has similar effects to those of IL-10 treatment. Moreover, costimulation through CD2/LFA-3 interaction is enough to induce proliferation and cytokine production in CD4+CD28null T-lymphocytes.

Abstract Image

IL-10 通过 LFA-3 和 HLA II 类抑制间接调节 CD4+CD28null T 淋巴细胞的功能活性。
慢性心力衰竭(CHF)患者常见 CD4+CD28 空 T 淋巴细胞扩增。其产生高水平促炎细胞因子的能力可能是这些细胞在 CHF 中的关键作用。IL-10 是限制 CD4+CD28 空 T 淋巴细胞反应的候选因子,而肿瘤坏死因子 (TNF) 则是与 CD28 表达丧失关系最密切的细胞因子。对 65 名慢性阻塞性肺病患者(平均年龄为 65.2 ± 13.84 岁)的血清 TNF 和 IL-10 水平进行了测定。IL-10/TNF比值≥1的患者,其CD4+CD28无效T淋巴细胞水平明显低于抗CD3刺激下CD28无效T淋巴细胞比值+CD28无效T淋巴细胞水平的患者。细胞因子抑制CD4+CD28无效T淋巴细胞产生TNF需要在抗CD3刺激前进行IL-10预处理。除了之前描述的IL-10对HLA-DR和ICAM-1表达的影响外,单核细胞中的LFA-3蛋白和mRNA水平在细胞因子存在时也会降低。IL-10 对 CD4+CD28null T 淋巴细胞的抑制作用可能是由 HLA II 类和 LFA-3 表达的减少介导的,因为阻断与这些成本刺激因子的相互作用与 IL-10 处理的效果相似。此外,通过 CD2/LFA-3 相互作用的成本刺激足以诱导 CD4+CD28null T 淋巴细胞增殖和产生细胞因子。
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来源期刊
Immunology
Immunology 医学-免疫学
CiteScore
11.90
自引率
1.60%
发文量
175
审稿时长
4-8 weeks
期刊介绍: Immunology is one of the longest-established immunology journals and is recognised as one of the leading journals in its field. We have global representation in authors, editors and reviewers. Immunology publishes papers describing original findings in all areas of cellular and molecular immunology. High-quality original articles describing mechanistic insights into fundamental aspects of the immune system are welcome. Topics of interest to the journal include: immune cell development, cancer immunology, systems immunology/omics and informatics, inflammation, immunometabolism, immunology of infection, microbiota and immunity, mucosal immunology, and neuroimmunology. The journal also publishes commissioned review articles on subjects of topical interest to immunologists, and commissions in-depth review series: themed sets of review articles which take a 360° view of select topics at the heart of immunological research.
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