New Skin-Relevant Cell Coculture Model With Stratum Corneum-Like Layer.

IF 2.6 3区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
BioMed Research International Pub Date : 2024-06-19 eCollection Date: 2024-01-01 DOI:10.1155/2024/1041392
Geovani Quijas, Agnieszka Lewińska, Marcin Łukaszewicz, Krzysztof Bojanowski
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Abstract

Two-dimensional (2D) cell culture is an important tool in the discovery of skin-active agents. Fibroblasts and keratinocytes, more rarely fibroblast-keratinocyte cocultures, are usually used for that purpose, where test compounds are added by mixing with the overlaying growth medium. However, such an approach is suboptimal because it lacks the stratum corneum component. The stratum corneum acts as a selective gatekeeper and opposes the intradermal permeation of many compounds that are bioactive when placed in direct contact with cells. One solution is to use reconstituted epidermis, but this approach is costly and time consuming. Here, a model is proposed, where the simplicity and convenience of the 2D cell culture is combined with the advantage of a hydrophobic barrier reminiscent of the skin horny layer. This model was tested with skin-relevant solvents, as well as with "naked" hydrophilic and encapsulated compounds. Cell viability and collagen stimulation were used as readouts. The results showed that the incorporation of a stratum corneum-substitute barrier on top of a 2D cell culture reduced the cytotoxicity of a common cosmetic solvent, dimethyl isosorbide (DMI), in cell culture and modified the bioactivity of the added actives (magnesium ascorbyl phosphate [MAP] and oligomeric proanthocyanidins [OPCs]/levan biopolymer), which became dependent on their ability to penetrate through a lipidic layer. Taken together, these results indicate a better physiological relevance of this cell culture model in workflows aimed at the discovery and analysis of skin-active compounds than conventional 2D systems.

带有类角质层的新型皮肤相关细胞共培养模型
二维(2D)细胞培养是发现皮肤活性物质的重要工具。为此,通常使用成纤维细胞和角质形成细胞,更少使用成纤维细胞-角质形成细胞共培养物,通过与覆盖的生长培养基混合来添加测试化合物。然而,这种方法并不理想,因为它缺少角质层成分。角质层起着选择性守门员的作用,当许多化合物与细胞直接接触时,会阻止其皮内渗透,而这些化合物具有生物活性。一种解决方案是使用重组表皮,但这种方法成本高且耗时。在这里,我们提出了一种模型,将二维细胞培养的简单方便与皮肤角质层疏水屏障的优势结合起来。我们使用与皮肤相关的溶剂以及 "裸 "亲水化合物和封装化合物对该模型进行了测试。细胞活力和胶原蛋白刺激被用作读数。结果表明,在二维细胞培养物顶部加入角质层替代屏障可降低常见化妆品溶剂二甲基异山梨醇(DMI)在细胞培养物中的细胞毒性,并改变添加活性物质(抗坏血酸磷酸镁[MAP]和低聚原花青素[OPCs]/levan 生物聚合物)的生物活性,这取决于它们穿透脂质层的能力。总之,这些结果表明,与传统的二维系统相比,这种细胞培养模型在发现和分析皮肤活性化合物的工作流程中具有更好的生理相关性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
BioMed Research International
BioMed Research International BIOTECHNOLOGY & APPLIED MICROBIOLOGY-MEDICINE, RESEARCH & EXPERIMENTAL
CiteScore
6.70
自引率
0.00%
发文量
1942
审稿时长
19 weeks
期刊介绍: BioMed Research International is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies covering a wide range of subjects in life sciences and medicine. The journal is divided into 55 subject areas.
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