André Schulz, Thorsten Knoll, Tino Jaeger, Ronan Le Harzic, Frank Stracke, Sascha L Wien, Yves Olsommer, Ina Meiser, Sylvia Wagner, Michael Rammensee, Oliver Kurz, Stephan Klesy, Loic Sermeus, Henrike Julich-Haertel, Yanick Schweitzer, Kai Januschowski, Thomas Velten, Peter Szurman
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引用次数: 0
Abstract
Purpose: To develop and evaluate a photovoltaic, wireless wide-field epiretinal prosthesis for the treatment of retinitis pigmentosa.
Methods: A mosaic array of thinned silicon-based photodiodes with integrated thin-film stimulation electrodes was fabricated with a flexible polyimide substrate film to form a film-based miniaturized electronic system with wireless optical power and signal transmission and integrated electrostimulation. Manufactured implants were characterized with respect to their optoelectronic performance and biocompatibility following DIN EN ISO 10993.
Results: A 14 mm diameter prosthesis containing 1276 pixels with a maximum sensitivity at a near infrared wavelength of 905 nm and maximized stimulation current density 30-50 μm below the electrodes was developed for direct activation of retinal ganglion cells during epiretinal stimulation. Fabricated prostheses demonstrated mucosal tolerance and the preservation of both metabolic activity, proliferation and membrane integrity of human fibroblasts as well as the retinal functions of bovine retinas. Illumination of the prosthesis, which was placed epiretinally on an isolated perfused bovine retina, with infrared light resulted in electrophysiological recordings reminiscent of an a-wave (hyperpolarization) and b-wave (depolarization).
Conclusions: A photovoltaic, wireless wide-field epiretinal prosthesis for the treatment of retinitis pigmentosa using near infrared light for signal transmission was designed, manufactured and its biocompatibility and functionality demonstrated in vitro and ex vivo.
目的：开发并评估用于治疗视网膜色素变性的光伏无线宽视场视网膜外假体：方法：用柔性聚酰亚胺基底薄膜制作了一个集成薄膜刺激电极的薄硅光电二极管镶嵌阵列，形成了一个具有无线光功率和信号传输以及集成电刺激功能的薄膜微型电子系统。根据 DIN EN ISO 10993 标准，对制造的植入物的光电性能和生物相容性进行了鉴定：开发了一种直径为 14 毫米的假体，包含 1276 个像素，在近红外波长 905 纳米时具有最大灵敏度，在电极下方 30-50 μm 处具有最大刺激电流密度，用于在视网膜外刺激过程中直接激活视网膜神经节细胞。制作的假体具有粘膜耐受性，并能保持人成纤维细胞的代谢活性、增殖和膜完整性，以及牛视网膜的视网膜功能。将假体置于离体灌注的牛视网膜上，用红外光对其进行照射，可产生令人联想到 a 波（超极化）和 b 波（去极化）的电生理记录：设计、制造了一种光电无线宽视场视网膜外假体，利用近红外线进行信号传输，用于治疗视网膜色素变性，并在体外和体内证明了其生物相容性和功能性。
期刊介绍：
Acta Ophthalmologica is published on behalf of the Acta Ophthalmologica Scandinavica Foundation and is the official scientific publication of the following societies: The Danish Ophthalmological Society, The Finnish Ophthalmological Society, The Icelandic Ophthalmological Society, The Norwegian Ophthalmological Society and The Swedish Ophthalmological Society, and also the European Association for Vision and Eye Research (EVER).
Acta Ophthalmologica publishes clinical and experimental original articles, reviews, editorials, educational photo essays (Diagnosis and Therapy in Ophthalmology), case reports and case series, letters to the editor and doctoral theses.
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