Strain sensor on a chip for quantifying the magnitudes of tensile stress on cells.

IF 7.3 1区 工程技术 Q1 INSTRUMENTS & INSTRUMENTATION
Microsystems & Nanoengineering Pub Date : 2024-06-25 eCollection Date: 2024-01-01 DOI:10.1038/s41378-024-00719-z
Yuyin Zhang, Yue Wang, Hongze Yin, Jiahao Wang, Na Liu, Songyi Zhong, Long Li, Quan Zhang, Tao Yue
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引用次数: 0

Abstract

During cardiac development, mechanotransduction from the in vivo microenvironment modulates cardiomyocyte growth in terms of the number, area, and arrangement heterogeneity. However, the response of cells to different degrees of mechanical stimuli is unclear. Organ-on-a-chip, as a platform for investigating mechanical stress stimuli in cellular mimicry of the in vivo microenvironment, is limited by the lack of ability to accurately quantify externally induced stimuli. However, previous technology lacks the integration of external stimuli and feedback sensors in microfluidic platforms to obtain and apply precise amounts of external stimuli. Here, we designed a cell stretching platform with an in-situ sensor. The in-situ liquid metal sensors can accurately measure the mechanical stimulation caused by the deformation of the vacuum cavity exerted on cells. The platform was applied to human cardiomyocytes (AC16) under cyclic strain (5%, 10%, 15%, 20 and 25%), and we found that cyclic strain promoted cell growth induced the arrangement of cells on the membrane to gradually unify, and stabilized the cells at 15% amplitude, which was even more effective after 3 days of culture. The platform's precise control and measurement of mechanical forces can be used to establish more accurate in vitro microenvironmental models for disease modeling and therapeutic research.

Abstract Image

用于量化细胞拉伸应力大小的芯片应变传感器。
在心脏发育过程中,来自体内微环境的机械传导调节了心肌细胞在数量、面积和排列异质性方面的生长。然而,细胞对不同程度机械刺激的反应尚不清楚。器官芯片作为研究细胞模拟体内微环境的机械应力刺激的平台,其局限性在于缺乏准确量化外部诱导刺激的能力。然而,以往的技术缺乏将外部刺激和反馈传感器整合到微流控平台中,以获取和应用精确的外部刺激量。在这里,我们设计了一种带有原位传感器的细胞拉伸平台。原位液态金属传感器可精确测量真空腔变形对细胞造成的机械刺激。我们将该平台应用于循环应变(5%、10%、15%、20% 和 25%)条件下的人类心肌细胞(AC16),发现循环应变促进细胞生长,诱导细胞在膜上的排列逐渐统一,并使细胞稳定在 15%的振幅,培养 3 天后效果更佳。该平台对机械力的精确控制和测量可用于建立更精确的体外微环境模型,用于疾病建模和治疗研究。
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来源期刊
Microsystems & Nanoengineering
Microsystems & Nanoengineering Materials Science-Materials Science (miscellaneous)
CiteScore
12.00
自引率
3.80%
发文量
123
审稿时长
20 weeks
期刊介绍: Microsystems & Nanoengineering is a comprehensive online journal that focuses on the field of Micro and Nano Electro Mechanical Systems (MEMS and NEMS). It provides a platform for researchers to share their original research findings and review articles in this area. The journal covers a wide range of topics, from fundamental research to practical applications. Published by Springer Nature, in collaboration with the Aerospace Information Research Institute, Chinese Academy of Sciences, and with the support of the State Key Laboratory of Transducer Technology, it is an esteemed publication in the field. As an open access journal, it offers free access to its content, allowing readers from around the world to benefit from the latest developments in MEMS and NEMS.
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