Differential expression of cellular prion protein (PrP^C) in mouse hepatitis virus induced neuroinflammation.

IF 2.3 4区医学 Q3 NEUROSCIENCES

Journal of NeuroVirology Pub Date : 2024-06-01 Epub Date: 2024-06-26 DOI:10.1007/s13365-024-01215-w

Satavisha Ghosh, Rishika Jana, Soumen Jana, Rahul Basu, Madhurima Chatterjee, Nishtha Ranawat, Jayasri Das Sarma

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Abstract

The cellular prion protein (PrP^C) is an extracellular cell membrane protein. Due to its diversified roles, a definite role of PrP^C has been difficult to establish. During viral infection, PrP^C has been reported to play a pleiotropic role. Here, we have attempted to envision the function of PrP^C in the neurotropic m-CoV-MHV-RSA59-induced model of neuroinflammation in C57BL/6 mice. A significant upregulation of PrP^C at protein and mRNA levels was evident in infected mouse brains during the acute phase of neuroinflammation. Furthermore, investigation of the effect of MHV-RSA59 infection on PrP^C expression in specific neuronal, microglial, and astrocytoma cell lines, revealed a differential expression of prion protein during neuroinflammation. Additionally, siRNA-mediated downregulation of prnp transcripts reduced the expression of viral antigen and viral infectivity in these cell lines. Cumulatively, our results suggest that PrP^C expression significantly increases during acute MHV-RSA59 infection and that PrP^C also assists in viral infectivity and viral replication.

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细胞朊病毒蛋白（PrPC）在小鼠肝炎病毒诱导的神经炎症中的差异表达。

细胞朊病毒蛋白（PrPC）是一种细胞外细胞膜蛋白。由于 PrPC 的作用多种多样，因此很难确定其确切的作用。据报道，在病毒感染过程中，PrPC 发挥着多重作用。在此，我们尝试设想 PrPC 在 m-CoV-MHV-RSA59 诱发的 C57BL/6 小鼠神经炎症模型中的功能。在神经炎症的急性期，受感染小鼠大脑中的 PrPC 蛋白和 mRNA 水平明显上调。此外，通过研究 MHV-RSA59 感染对特定神经元、小胶质细胞和星形细胞瘤细胞系中 PrPC 表达的影响，发现神经炎症期间朊病毒蛋白的表达存在差异。此外，siRNA 介导的 prnp 转录本下调降低了这些细胞系的病毒抗原表达和病毒传染性。综上所述，我们的研究结果表明，在 MHV-RSA59 急性感染期间，PrPC 的表达明显增加，PrPC 还有助于病毒的感染性和病毒复制。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of NeuroVirology 医学-病毒学

CiteScore

6.60

自引率

3.10%

发文量

审稿时长

6-12 weeks

期刊介绍： The Journal of NeuroVirology (JNV) provides a unique platform for the publication of high-quality basic science and clinical studies on the molecular biology and pathogenesis of viral infections of the nervous system, and for reporting on the development of novel therapeutic strategies using neurotropic viral vectors. The Journal also emphasizes publication of non-viral infections that affect the central nervous system. The Journal publishes original research articles, reviews, case reports, coverage of various scientific meetings, along with supplements and special issues on selected subjects. The Journal is currently accepting submissions of original work from the following basic and clinical research areas: Aging & Neurodegeneration, Apoptosis, CNS Signal Transduction, Emerging CNS Infections, Molecular Virology, Neural-Immune Interaction, Novel Diagnostics, Novel Therapeutics, Stem Cell Biology, Transmissable Encephalopathies/Prion, Vaccine Development, Viral Genomics, Viral Neurooncology, Viral Neurochemistry, Viral Neuroimmunology, Viral Neuropharmacology.