Mice fatal pneumonia model induced by less-virulent Streptococcus pneumoniae via intratracheal aerosolization.

IF 2.5 4区生物学 Q3 MICROBIOLOGY

Future microbiology Pub Date : 2024-08-12 Epub Date: 2024-06-24 DOI:10.1080/17460913.2024.2355738

Jiazhen Wang, Lingfei Hu, Zhijun Zhang, Chengyu Sui, Xiaoyu Zhu, Chengxi Wu, Lili Zhang, Meng Lv, Wenhui Yang, Dongsheng Zhou, Zhengling Shang

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引用次数: 0

Abstract

Aim: Animal models of fatal pneumonia caused by Streptococcus pneumoniae (Spn) have not been reliably generated using many strains of less virulent serotypes.Materials & methods: Pulmonary infection of a less virulent Spn serotype1 strain in the immunocompetent mice was established via the intratracheal aerosolization (ITA) route. The survival, local and systemic bacterial spread, pathological changes and inflammatory responses of this model were compared with those of mice challenged via the intratracheal instillation, intranasal instillation and intraperitoneal injection routes.Results: ITA and intratracheal instillation both induced fatal pneumonia; however, ITA resulted in better lung bacterial deposition and distribution, pathological homogeneity and delivery efficiency.Conclusion: ITA is an optimal route for developing animal models of severe pulmonary infections.

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通过气管内雾化吸入低毒性肺炎链球菌诱发小鼠致命肺炎模型。

目的：肺炎链球菌（Spn）引起的致命性肺炎的动物模型尚未可靠地使用许多毒力较弱的血清型菌株来制作。材料与方法：通过气管内雾化（ITA）途径在免疫功能正常的小鼠体内建立毒力较弱的 Spn 血清型 1 菌株的肺部感染。将该模型的存活率、局部和全身细菌扩散、病理变化和炎症反应与通过气管内灌注、鼻内灌注和腹腔注射途径感染的小鼠进行比较。结果ITA和气管内灌注均可诱发致命性肺炎，但ITA在肺部细菌沉积和分布、病理均匀性和输送效率方面更胜一筹。结论ITA是建立严重肺部感染动物模型的最佳途径。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Future microbiology 生物-微生物学

CiteScore

4.90

自引率

3.20%

发文量

134

审稿时长

6-12 weeks

期刊介绍： Future Microbiology delivers essential information in concise, at-a-glance article formats. Key advances in the field are reported and analyzed by international experts, providing an authoritative but accessible forum for this increasingly important and vast area of research.