Evaluating patient immunocompetence through antibody response to pneumococcal polysaccharide vaccine using a newly developed 23 serotype multiplexed assay

IF 4.5 3区 医学 Q2 IMMUNOLOGY
Thomas B. Martins , Harry R. Hill , Lisa K. Peterson
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Abstract

Assessing T-cell independent antibody response to polysaccharide vaccines is crucial for diagnosing humoral immune deficiencies. However, immunocompetence criteria based on S. pneumoniae vaccination remain unclear. We evaluated IgG antibody vaccine response in healthy individuals to establish interpretive criteria. Pre- and 4-week post-vaccination sera were collected from 79 adults. Antibody concentrations to PNEUMOVAX 23 serotypes were measured using a multiplexed platform. Immunocompetence was determined by fold increase in post-vaccination response, percentage of serotypes achieving 4- or 2-fold antibody ratio, and post-vaccination concentration ≥ 1.3 μg/mL. Immunogenicity varied widely across the 23 serotypes (26.6% to 94.9% for ≥4-fold increase, 51.9% to 98.7% for ≥2-fold increase). Immunocompetence based on historic criteria of ≥4-fold increase in antibody ratio to ≥70% of serotypes was low (72.2%), but increased to 98.7% with criteria of at least a 2-fold increase and/or post-vaccination concentration ≥ 1.3 μg/mL. Current criteria for assessing immunocompetence may be overly stringent and require updating.

使用新开发的 23 种血清型多重检测法,通过肺炎球菌多糖疫苗抗体反应评估患者的免疫能力。
评估独立于 T 细胞的多糖疫苗抗体反应对于诊断体液免疫缺陷至关重要。然而,基于肺炎双球菌疫苗接种的免疫能力标准仍不明确。我们评估了健康人的 IgG 抗体疫苗反应,以建立解释性标准。我们收集了 79 名成年人接种前和接种后 4 周的血清。使用多重平台测定了 PNEUMOVAX 23 种血清型的抗体浓度。免疫能力根据接种后反应的增加倍数、达到 4 倍或 2 倍抗体比率的血清型百分比以及接种后浓度≥ 1.3 μg/mL来确定。23 种血清型的免疫原性差异很大(≥4 倍抗体增加率为 26.6% 至 94.9%,≥2 倍抗体增加率为 51.9% 至 98.7%)。根据历史标准,对≥70%血清型的抗体比率增加≥4倍的免疫能力较低(72.2%),但根据至少增加2倍和/或接种后浓度≥1.3微克/毫升的标准,免疫能力增加到98.7%。目前评估免疫能力的标准可能过于严格,需要更新。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Clinical immunology
Clinical immunology 医学-免疫学
CiteScore
12.30
自引率
1.20%
发文量
212
审稿时长
34 days
期刊介绍: Clinical Immunology publishes original research delving into the molecular and cellular foundations of immunological diseases. Additionally, the journal includes reviews covering timely subjects in basic immunology, along with case reports and letters to the editor.
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