Genome editing using CRISPR, CAST, and Fanzor systems

IF 3.7 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Molecules and Cells Pub Date : 2024-07-01 DOI:10.1016/j.mocell.2024.100086

Beomjong Song , Sangsu Bae

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引用次数: 0

Abstract

Genetic engineering technologies are essential not only for basic science but also for generating animal models for therapeutic applications. The clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated protein (Cas) system, derived from adapted prokaryotic immune responses, has led to unprecedented advancements in the field of genome editing because of its ability to precisely target and edit genes in a guide RNA-dependent manner. The discovery of various types of CRISPR-Cas systems, such as CRISPR-associated transposons (CASTs), has resulted in the development of novel genome editing tools. Recently, research has expanded to systems associated with obligate mobile element guided activity (OMEGA) RNAs, including ancestral CRISPR-Cas and eukaryotic Fanzor systems, which are expected to complement the conventional CRISPR-Cas systems. In this review, we briefly introduce the features of various CRISPR-Cas systems and their application in diverse animal models.

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使用 CRISPR、CAST 和 Fanzor 系统进行基因组编辑。

基因工程技术不仅对基础科学至关重要，而且对制作治疗用动物模型也至关重要。簇状规则间距短回文重复序列（CRISPR）-CRISPR相关蛋白（Cas）系统源于适应性原核生物免疫反应，因其能够以依赖引导RNA的方式精确靶向和编辑基因，在基因组编辑领域取得了前所未有的进展。各种类型的 CRISPR-Cas 系统，如 CRISPR 相关转座子（CAST）的发现，促进了新型基因组编辑工具的开发。最近，研究已扩展到与欧米伽 RNA 相关的系统，包括祖先 CRISPR-Cas 和真核 Fanzor 系统，这些系统有望成为传统 CRISPR-Cas 系统的补充。在这篇综述中，我们将简要介绍各种 CRISPR-Cas 系统的特点及其在不同动物模型中的应用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Molecules and Cells 生物-生化与分子生物学

CiteScore

6.60

自引率

10.50%

发文量

审稿时长

2.3 months

期刊介绍： Molecules and Cells is an international on-line open-access journal devoted to the advancement and dissemination of fundamental knowledge in molecular and cellular biology. It was launched in 1990 and ISO abbreviation is ''Mol. Cells''. Reports on a broad range of topics of general interest to molecular and cell biologists are published. It is published on the last day of each month by the Korean Society for Molecular and Cellular Biology.

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