Comparative Evaluation of Multiplex PCR, RLEP PCR and LAMP PCR in Urine, Stool and Blood Samples for the Diagnosis of Pediatric Leprosy - A Cross-Sectional Study.

IF 1.7 4区医学 Q2 PEDIATRICS

Indian pediatrics Pub Date : 2024-07-15 Epub Date: 2024-06-13

Shivam Sharma, Rajeshwar Dayal, Raj Kamal, Dharmendra Singh, Shripad A Patil, Neeraj Kumar, Sheo Pratap Singh, Madhu Nayak

{"title":"Comparative Evaluation of Multiplex PCR, RLEP PCR and LAMP PCR in Urine, Stool and Blood Samples for the Diagnosis of Pediatric Leprosy - A Cross-Sectional Study.","authors":"Shivam Sharma, Rajeshwar Dayal, Raj Kamal, Dharmendra Singh, Shripad A Patil, Neeraj Kumar, Sheo Pratap Singh, Madhu Nayak","doi":"","DOIUrl":null,"url":null,"abstract":"Objective: To compare the diagnostic efficacy of multiplex polymerase chain reaction (PCR), Mycobacterium leprae-specific repetitive element (RLEP) PCR and loop-mediated isothermal amplification (LAMP) PCR in the diagnosis of pediatric leprosy as an alternative to slit-skin smear (SSS) examination.Methods: A cross-sectional study was performed on 26 children aged 0-18 years with characteristic skin lesions of leprosy. SSS examination for acid fast bacilli (AFB) was performed for all children. Additionally, urine, stool and blood samples were tested by three PCR techniques - multiplex, RLEP and LAMP. The results of these tests were compared with each other and with results of SSS examination for acid fast bacilli (AFB) using appropriate statistical tests.Results: Out of 26 patients studied, SSS examination was positive for AFB in 7 cases (26.9%). In blood samples, the positivity of multiplex PCR, RLEP PCR and LAMP PCR was 84.6%, 80.8%, and 80.8%, respectively. Multiplex PCR in blood samples was positive in 100% (n = 7) of SSS positive cases and 84.2% (16 out of 19) of the SSS negative cases (P < 0.001). The positivity of all PCR methods in urine and stool samples was significantly lesser than in blood.Conclusion: Multiplex PCR in blood sample is a superior diagnostic tool for pediatric leprosy compared to RLEP PCR and LAMP PCR as well as SSS examination.","PeriodicalId":13291,"journal":{"name":"Indian pediatrics","volume":" ","pages":"661-665"},"PeriodicalIF":1.7000,"publicationDate":"2024-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Indian pediatrics","FirstCategoryId":"3","ListUrlMain":"","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/6/13 0:00:00","PubModel":"Epub","JCR":"Q2","JCRName":"PEDIATRICS","Score":null,"Total":0}

引用次数: 0

Abstract

Objective: To compare the diagnostic efficacy of multiplex polymerase chain reaction (PCR), Mycobacterium leprae-specific repetitive element (RLEP) PCR and loop-mediated isothermal amplification (LAMP) PCR in the diagnosis of pediatric leprosy as an alternative to slit-skin smear (SSS) examination.

Methods: A cross-sectional study was performed on 26 children aged 0-18 years with characteristic skin lesions of leprosy. SSS examination for acid fast bacilli (AFB) was performed for all children. Additionally, urine, stool and blood samples were tested by three PCR techniques - multiplex, RLEP and LAMP. The results of these tests were compared with each other and with results of SSS examination for acid fast bacilli (AFB) using appropriate statistical tests.

Results: Out of 26 patients studied, SSS examination was positive for AFB in 7 cases (26.9%). In blood samples, the positivity of multiplex PCR, RLEP PCR and LAMP PCR was 84.6%, 80.8%, and 80.8%, respectively. Multiplex PCR in blood samples was positive in 100% (n = 7) of SSS positive cases and 84.2% (16 out of 19) of the SSS negative cases (P < 0.001). The positivity of all PCR methods in urine and stool samples was significantly lesser than in blood.

Conclusion: Multiplex PCR in blood sample is a superior diagnostic tool for pediatric leprosy compared to RLEP PCR and LAMP PCR as well as SSS examination.

本刊更多论文

尿液、粪便和血液样本中多重 PCR、RLEP PCR 和 LAMP PCR 用于诊断小儿麻风病的比较评估--一项横断面研究。

目的比较多重聚合酶链反应（PCR）、麻风分枝杆菌特异性重复元件聚合酶链反应（RLEP）和环介导等温扩增聚合酶链反应（LAMP）在诊断小儿麻风病中替代裂隙皮肤涂片检查（SSS）的诊断效果：方法：对 26 名 0-18 岁具有麻风病特征性皮损的儿童进行了横断面研究。对所有儿童进行了酸性快速杆菌（AFB）SSS检查。此外，还采用多重、RLEP 和 LAMP 三种 PCR 技术对尿液、粪便和血液样本进行了检测。使用适当的统计检验将这些检验结果相互比较，并与 SSS 酸性快速杆菌（AFB）检查结果进行比较：在所研究的 26 例患者中，7 例（26.9%）的 SSS 检查结果呈 AFB 阳性。在血液样本中，多重 PCR、RLEP PCR 和 LAMP PCR 的阳性率分别为 84.6%、80.8% 和 80.8%。在血液样本中，SSS 阳性病例的多重 PCR 阳性率为 100%（7 例），SSS 阴性病例的多重 PCR 阳性率为 84.2%（19 例中有 16 例）（P < 0.001）。尿液和粪便样本中所有 PCR 方法的阳性率都明显低于血液样本：结论：与RLEP PCR和LAMP PCR以及SSS检查相比，血液样本中的多重PCR是一种更好的小儿麻风病诊断工具。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Indian pediatrics 医学-小儿科

CiteScore

3.30

自引率

8.70%

发文量

344

审稿时长

3-8 weeks

期刊介绍： The general objective of Indian Pediatrics is "To promote the science and practice of Pediatrics." An important guiding principle has been the simultaneous need to inform, educate and entertain the target audience. The specific key objectives are: -To publish original, relevant, well researched peer reviewed articles on issues related to child health. -To provide continuing education to support informed clinical decisions and research. -To foster responsible and balanced debate on controversial issues that affect child health, including non-clinical areas such as medical education, ethics, law, environment and economics. -To achieve the highest level of ethical medical journalism and to produce a publication that is timely, credible and enjoyable to read.