Placental neutrophil reverse trans-migration and maternal serum neutrophil extracellular trap expression in HIV infection co-morbid pre-eclampsia in women of African ancestry.

IF 2.1 4区 生物学 Q4 CELL BIOLOGY
Histochemistry and Cell Biology Pub Date : 2024-10-01 Epub Date: 2024-06-24 DOI:10.1007/s00418-024-02298-6
Merantha Moodley, Jagidesa Moodley, Thajasvarie Naicker
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引用次数: 0

Abstract

Neutrophil extracellular traps (NETs) and placental neutrophil reverse transmigration (r-TM) are implicated in the pathogenesis of pre-eclampsia (PE). However, the role of the comorbidity of PE and human immunodeficiency virus (HIV) infection in placental neutrophil r-TM and serum NETs remains unknown. Human placental tissue (n = 160) and serum (n = 80) samples were obtained post-ethical approval and divided by pregnancy type and HIV status and across the study population. Immunohistochemistry and morphometry were performed to localize and quantify junctional adhesion molecule-C (JAM-C) expression as an inverse marker of neutrophil r-TM within placental villi. An enzyme-linked immunosorbent assay (ELISA) was performed to quantify the concentration of citrullinated histone H3 (cit-H3) as a marker of NETs. GraphPad Prism (version 8.0.2) was used to compare the results, and a p value of p < 0.05 was considered statistically significant. The localization of JAM-C was observed on the syncytiotrophoblasts (STBs) and endothelial cells of placental villi. The immunoexpression of JAM-C was elevated in PE vs. normotensive (N) placentae. In the exchange villi, JAM-C immunoexpression was higher in the N+ve vs. N-ve group. However, in PE comorbid HIV infection, JAM-C expression was lower in the PE+ve vs. PE-ve group. Citrullinated histone-H3 concentration was lower in the N+ve vs. N-ve group but elevated in early-onset PE (EOPE)+ve vs. late-onset PE (LOPE)+ve group. These results indicate that PE and HIV-infected placentae individually express elevated JAM-C, manifesting in less neutrophil r-TM. However, in exchange villi of PE comorbid with HIV infection reduced JAM-C enhances neutrophil r-TM, thus supporting the synergistic effect of PE comorbid with HIV.

Abstract Image

非洲裔妇女感染艾滋病病毒合并先兆子痫时胎盘中性粒细胞反向迁移和母体血清中性粒细胞胞外捕获器的表达。
中性粒细胞胞外捕获物(NET)和胎盘中性粒细胞反向迁移(r-TM)与先兆子痫(PE)的发病机制有关。然而,子痫前期和人类免疫缺陷病毒(HIV)感染并发症在胎盘中性粒细胞反向迁移(r-TM)和血清中性粒细胞NETs中的作用仍不清楚。人类胎盘组织(n = 160)和血清(n = 80)样本是在伦理批准后获得的,并按妊娠类型、HIV 感染状况和研究人群进行了划分。通过免疫组化和形态计量学方法,对作为胎盘绒毛内中性粒细胞r-TM反向标记的交界粘附分子-C(JAM-C)表达进行定位和量化。用酶联免疫吸附试验(ELISA)定量检测作为NET标记物的瓜氨酸化组蛋白H3(cit-H3)的浓度。使用 GraphPad Prism(8.0.2 版)对结果进行比较,P 值为 p
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来源期刊
Histochemistry and Cell Biology
Histochemistry and Cell Biology 生物-细胞生物学
CiteScore
4.90
自引率
8.70%
发文量
112
审稿时长
1 months
期刊介绍: Histochemistry and Cell Biology is devoted to the field of molecular histology and cell biology, publishing original articles dealing with the localization and identification of molecular components, metabolic activities and cell biological aspects of cells and tissues. Coverage extends to the development, application, and/or evaluation of methods and probes that can be used in the entire area of histochemistry and cell biology.
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