Novel strategy for Poxviridae prevention: Thermostable combined subunit vaccine patch with intense immune response

IF 4.5 2区医学 Q1 PHARMACOLOGY & PHARMACY

Antiviral research Pub Date : 2024-06-21 DOI:10.1016/j.antiviral.2024.105943

Yuan Wen , Shuyue Deng , Tianmin Wang , Mengtian Gao , Wenlong Nan , Fang Tang , Qinghong Xue , Yanmin Ju , Jianjun Dai , Yurong Wei , Feng Xue

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引用次数: 0

Abstract

Poxviruses gained international attention due to the sharp rise in monkeypox cases in recent years, highlighting the urgent need for the development of a secure and reliable vaccine. This study involved the development of an innovative combined subunit vaccine (CSV) targeting poxviruses, with lumpy skin disease virus (LSDV) serving as the model virus. To this end, the potential sites for poxvirus vaccines were fully evaluated to develop and purify four recombinant proteins. These proteins were then successfully delivered to the dermis in a mouse model by utilizing dissolvable microneedle patches (DMPs). This approach simplified the vaccination procedure and significantly mitigated the associated risk. CSV-loaded DMPs contained four recombinant proteins and a novel adjuvant, CpG, which allowed DMPs to elicit the same intensity of humoral and cellular immunity as subcutaneous injection. Following immunization with SC and DMP, the mice exhibited notable levels of neutralizing antibodies, albeit at a low concentration. It is noteworthy that the CSV loaded into DMPs remained stable for at least 4 months at room temperature, effectively addressing the storage and transportation challenges. Based on the study findings, CSV-loaded DMPs are expected to be utilized worldwide as an innovative technique for poxvirus inoculation, especially in underdeveloped regions. This novel strategy is crucial for the development of future poxvirus vaccines.

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预防痘病毒的新策略：可产生强烈免疫反应的热稳定性联合亚单位疫苗贴片。

由于近年来猴痘病例的急剧上升，痘病毒受到了国际社会的关注，这凸显了开发安全可靠疫苗的迫切性。本研究以块状皮肤病病毒（LSDV）为模型病毒，针对痘病毒开发了一种创新的联合亚单位疫苗（CSV）。为此，对痘病毒疫苗的潜在接种位点进行了全面评估，以开发和纯化四种重组蛋白。然后利用可溶解微针贴片（DMPs）成功地将这些蛋白输送到小鼠模型的真皮层。这种方法简化了疫苗接种程序，大大降低了相关风险。装载CSV的DMPs含有四种重组蛋白和一种新型佐剂CpG，这使得DMPs能引起与皮下注射相同强度的体液免疫和细胞免疫。用 SC 和 DMP 进行免疫后，小鼠表现出明显的中和抗体水平，尽管浓度较低。值得注意的是，载入 DMP 的 CSV 在室温下至少能保持稳定 4 个月，有效解决了储存和运输难题。根据研究结果，作为一种创新的痘病毒接种技术，载入 CSV 的 DMPs 预计将在全球范围内得到应用，尤其是在欠发达地区。这种新型策略对未来痘病毒疫苗的开发至关重要。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Antiviral research 医学-病毒学

CiteScore

17.10

自引率

3.90%

发文量

157

审稿时长

34 days

期刊介绍： Antiviral Research is a journal that focuses on various aspects of controlling viral infections in both humans and animals. It is a platform for publishing research reports, short communications, review articles, and commentaries. The journal covers a wide range of topics including antiviral drugs, antibodies, and host-response modifiers. These topics encompass their synthesis, in vitro and in vivo testing, as well as mechanisms of action. Additionally, the journal also publishes studies on the development of new or improved vaccines against viral infections in humans. It delves into assessing the safety of drugs and vaccines, tracking the evolution of drug or vaccine-resistant viruses, and developing effective countermeasures. Another area of interest includes the identification and validation of new drug targets. The journal further explores laboratory animal models of viral diseases, investigates the pathogenesis of viral diseases, and examines the mechanisms by which viruses avoid host immune responses.