Histological and Transcriptomic Insights into the Ovary Development of Hemibarbus labeo Injected with Spawn-Inducing Hormones

IF 2.6 3区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Xinming Gao, Yaoping Lv, Qingmin Dai, Ling Zhu, Siqi Liu, Zehui Hu, Junkai Lu, Haidong Zhou, Zufei Mei
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Abstract

Naturally, the ovaries of many farmed fish can only develop to stage IV (mainly including stage IV oocytes, known as full-grown postvitellogenic oocytes). Therefore, spawn-inducing hormone injections are used to promote ovary development and oocyte maturation, facilitating reproduction in the aquaculture industry. The study of spawn-inducing hormones and their underlying neuroendocrine mechanisms has been a recent focus in fish reproductive biology. However, the intra-ovarian regulatory mechanisms of ovary development and oocyte maturation after hormone injection require further investigation. In this study, we explored the histological and transcriptomic map of the ovary of Hemibarbus labeo after hormone injection to reveal changes in the ovary. The gonad index significantly increased after hormone injection for 5.5 h, after which no significant change was observed. Histological analysis showed that the nuclei had moved to one side of the oocytes at 5.5 h after hormone injection. Moreover, the volume of the oocytes increased and their yolk membranes thickened. Oocytes then underwent their first meiotic division at 5.5–11 h after hormone injection. Subsequently, the follicular membrane was ruptured, and ovulation was completed at 11–16.5 h after hormone injection. In addition, we identified 3189 differentially expressed genes (DEGs) on comparing the transcriptomes at different time points after hormone injection. These DEGs were significantly enriched in the GO terms of nervous system process, molecular transducer activity, and extracellular region, and the KEGG pathways of TNF signaling and cytokine–cytokine receptor interaction; these may play important roles in ovary development and oocyte maturation. Within these pathways, genes such as apoe, creb3, jun, junb, il11, and il8 may play important roles in steroid hormone synthesis and ovulation. Conclusively, our results show detailed sequential dynamics of oocyte development and provide new insights into the intra-ovarian regulatory mechanisms of ovarian development and oocyte maturation in H. labeo. These findings may be important for research on improving egg quality and reproduction in aquaculture.

Abstract Image

通过组织学和转录组学深入了解注射产卵诱导激素的唇鲵卵巢发育过程
自然,许多养殖鱼类的卵巢只能发育到第四阶段(主要包括第四阶段的卵母细胞,即完全成熟的卵裂后卵母细胞)。因此,注射催产素可促进卵巢发育和卵母细胞成熟,促进水产养殖业的繁殖。对催产激素及其潜在的神经内分泌机制的研究是近年来鱼类生殖生物学的一个重点。然而,激素注射后卵巢内对卵巢发育和卵母细胞成熟的调控机制还需要进一步研究。在本研究中,我们探索了注射激素后唇姬蛙卵巢的组织学和转录组图谱,以揭示卵巢的变化。注射激素5.5小时后,性腺指数明显增加,之后没有观察到明显变化。组织学分析表明,注射激素 5.5 小时后,卵母细胞核向一侧移动。此外,卵母细胞体积增大,卵黄膜增厚。然后,卵母细胞在注射激素后 5.5-11 小时进行了第一次减数分裂。随后,卵泡膜破裂,并在激素注射后 11-16.5 h 完成排卵。此外,通过比较激素注射后不同时间点的转录组,我们发现了3189个差异表达基因(DEGs)。这些DEGs明显富集于神经系统过程、分子换能器活性和细胞外区域等GO术语,以及TNF信号转导和细胞因子-细胞因子受体相互作用等KEGG通路,它们可能在卵巢发育和卵母细胞成熟过程中发挥重要作用。在这些通路中,apoe、creb3、jun、junb、il11 和 il8 等基因可能在类固醇激素合成和排卵中发挥重要作用。总之,我们的研究结果显示了卵母细胞发育的详细顺序动态变化,并对拉贝鱼卵巢发育和卵母细胞成熟的卵巢内调控机制提供了新的见解。这些发现可能对提高水产养殖中卵子质量和繁殖能力的研究具有重要意义。
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来源期刊
Marine Biotechnology
Marine Biotechnology 工程技术-海洋与淡水生物学
CiteScore
4.80
自引率
3.30%
发文量
95
审稿时长
2 months
期刊介绍: Marine Biotechnology welcomes high-quality research papers presenting novel data on the biotechnology of aquatic organisms. The journal publishes high quality papers in the areas of molecular biology, genomics, proteomics, cell biology, and biochemistry, and particularly encourages submissions of papers related to genome biology such as linkage mapping, large-scale gene discoveries, QTL analysis, physical mapping, and comparative and functional genome analysis. Papers on technological development and marine natural products should demonstrate innovation and novel applications.
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