The Influence of Bioceramic Cements on the Activity of Macrophages of Different Lineages: A Laboratory Study

IF 3.5 2区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of endodontics Pub Date : 2024-10-01 DOI:10.1016/j.joen.2024.06.008

{"title":"The Influence of Bioceramic Cements on the Activity of Macrophages of Different Lineages: A Laboratory Study","authors":"","doi":"10.1016/j.joen.2024.06.008","DOIUrl":null,"url":null,"abstract":"<div><h3>Introduction</h3><div>The repair process of periradicular tissues depends, among other factors, on the properties of endodontic cements. Macrophages are among the main cells involved in this process.</div></div><div><h3>Materials and Methods</h3><div>Murine peritoneal macrophages obtained from C57BL/6 (MBL6) and BALB/c (MBalb) mice, respectively, were cultured with capillaries containing or not Endosequence BC Sealer (BC), Sealer Plus BC (MK), Bio-C Sealer (Ang), and mineral trioxide aggregate (MTA). Cell viability was measured by Trypan blue and MTT methods at 24, 48, and 72 hours. Cell adhesion, phagocytosis of <em>Saccharomyces boulardii</em>, production of reactive oxygen species (ROS), nitric oxide (NO), and the cytokines tumor necrosis factor-α and transforming growth factor (TGF)-β were also evaluated. The data were analyzed using the analysis of variance test (<em>P</em> < .05).</div></div><div><h3>Results</h3><div>Cell viability was similar between bioceramic sealers and MTA (<em>P</em> > .05). There was no statistical difference between both macrophages when adherence and phagocytose were assayed. The presence of inflammation stimulus significantly altered the production of ROS by MBL6 macrophages in contact with the cements. The production of TGF-β was similar for both lineages of macrophages.</div></div><div><h3>Conclusions</h3><div>This study shows that the evaluated bioceramic cements do not interfere with MBL6 and MBalb macrophage adhesion, phagocytic capacity, or TGF-β production. The cements stimulated the production of ROS by MBL6 macrophages in response to induced inflammation, potentially favoring the elimination of residual pathogens.</div></div>","PeriodicalId":15703,"journal":{"name":"Journal of endodontics","volume":null,"pages":null},"PeriodicalIF":3.5000,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of endodontics","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0099239924003595","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"DENTISTRY, ORAL SURGERY & MEDICINE","Score":null,"Total":0}

引用次数: 0

Abstract

Introduction

The repair process of periradicular tissues depends, among other factors, on the properties of endodontic cements. Macrophages are among the main cells involved in this process.

Materials and Methods

Murine peritoneal macrophages obtained from C57BL/6 (MBL6) and BALB/c (MBalb) mice, respectively, were cultured with capillaries containing or not Endosequence BC Sealer (BC), Sealer Plus BC (MK), Bio-C Sealer (Ang), and mineral trioxide aggregate (MTA). Cell viability was measured by Trypan blue and MTT methods at 24, 48, and 72 hours. Cell adhesion, phagocytosis of Saccharomyces boulardii, production of reactive oxygen species (ROS), nitric oxide (NO), and the cytokines tumor necrosis factor-α and transforming growth factor (TGF)-β were also evaluated. The data were analyzed using the analysis of variance test (P < .05).

Results

Cell viability was similar between bioceramic sealers and MTA (P > .05). There was no statistical difference between both macrophages when adherence and phagocytose were assayed. The presence of inflammation stimulus significantly altered the production of ROS by MBL6 macrophages in contact with the cements. The production of TGF-β was similar for both lineages of macrophages.

Conclusions

This study shows that the evaluated bioceramic cements do not interfere with MBL6 and MBalb macrophage adhesion, phagocytic capacity, or TGF-β production. The cements stimulated the production of ROS by MBL6 macrophages in response to induced inflammation, potentially favoring the elimination of residual pathogens.

查看原文本刊更多论文

生物陶瓷水泥对不同血统巨噬细胞活性的影响：一项实验室研究。

导言：根尖周组织的修复过程主要取决于牙髓胶结物的特性。参与这一过程的主要细胞之一是巨噬细胞：将分别取自 C57BL/6 (MBL6) 和 BALB/c (MBalb) 小鼠的小鼠腹腔巨噬细胞与含有或不含有 Endosequence BC Sealer® (BC)、Sealer Plus BC® (MK)、Bio C Sealer (Ang) 和 MTA® 的毛细血管一起培养。细胞活力在 24、48 和 72 小时内通过胰蓝法和 MTT 法进行测量。此外，还评估了细胞粘附性、布拉氏酵母菌的吞噬作用、活性氧（ROS）、一氧化氮（NO）的产生以及细胞因子 TNF-α 和 TGF-β。数据分析采用方差分析（pResults）：生物陶瓷封闭剂和 MTA 的细胞活力相似（p>0.05）。在检测巨噬细胞的粘附性和吞噬能力时，两者之间没有统计学差异。炎症刺激的存在明显改变了与水泥接触的 MBL6 巨噬细胞产生的 ROS。两系巨噬细胞产生的 TGF-β 相似：这项研究表明，所评估的生物陶瓷水门汀不会干扰 MBL6 和 MBalb 巨噬细胞的粘附、吞噬能力以及 TGF-β 的产生。在诱发炎症时，这些粘合剂会刺激 MBL6 巨噬细胞产生 ROS，从而有可能促进残留病原体的清除。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of endodontics 医学-牙科与口腔外科

CiteScore

8.80

自引率

9.50%

发文量

224

审稿时长

42 days

期刊介绍： The Journal of Endodontics, the official journal of the American Association of Endodontists, publishes scientific articles, case reports and comparison studies evaluating materials and methods of pulp conservation and endodontic treatment. Endodontists and general dentists can learn about new concepts in root canal treatment and the latest advances in techniques and instrumentation in the one journal that helps them keep pace with rapid changes in this field.