DWARF TILLER1 regulates apical-basal pattern formation and proper orientation of rice embryos.

IF 6.5 1区生物学 Q1 PLANT SCIENCES

Plant Physiology Pub Date : 2024-09-02 DOI:10.1093/plphys/kiae318

Jingyao Tang, Xiaorong Huang, Mengxiang Sun, Wanqi Liang

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引用次数: 0

Abstract

Body axis establishment is one of the earliest patterning events in plant embryogenesis. Asymmetric zygote division is critical for apical-basal axis formation in Arabidopsis (Arabidopsis thaliana). However, how the orientation of the cell division plane is regulated and its relation to apical-basal axis establishment and proper position of embryos in grasses remain poorly understood. By characterizing mutants of 3 rice (Oryza sativa) WUSCHEL HOMEOBOX9 (WOX9) genes, whose paralogs in Arabidopsis play essential roles in zygotic asymmetric cell division and cell fate determination, we found 2 kinds of independent embryonic defects: topsy-turvy embryos, in which apical-basal axis twists from being parallel to the longitudinal axis of the seed to being perpendicular; and organ-less embryos. In contrast to their Arabidopsis orthologs, OsWOX9s displayed dynamic distribution during embryo development. Both DWT1/OsWOX9A and DWL2/WOX9C play major roles in the apical-basal axis formation and initiation of stem cells. In addition, DWT1 has a distinct function in regulating the first few embryonic cell divisions to ensure the correct orientation of the embryo in the ovary. In summary, DWT1 acts in 2 steps during rice embryo pattern formation: the initial zygotic division, and with DWL2 to establish the main body axes and stem cell fate 2 to 3 d after pollination.

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DWARF TILLER1调控水稻胚胎顶端-基部模式的形成和正确定向。

体轴的建立是植物胚胎发生过程中最早的模式化事件之一。在拟南芥（Arabidopsis thaliana）中，不对称的子实体分裂对于顶轴-基轴的形成至关重要。然而，人们对禾本科植物细胞分裂平面的方向如何调节及其与顶基轴的建立和胚胎的正确位置之间的关系仍然知之甚少。拟南芥中的WUSCHEL HOMEOBOX9（WOX9）基因的旁系亲属在合子不对称细胞分裂和细胞命运决定中起着重要作用，通过对3个水稻（Oryza sativa）WUSCHEL HOMEOBOX9（WOX9）基因的突变体进行表征，我们发现了2种独立的胚胎缺陷：颠倒胚（胚的顶基轴从平行于种子纵轴扭曲为垂直）和无器官胚。与拟南芥同源物相比，OsWOX9s 在胚胎发育过程中表现出动态分布。DWT1/OsWOX9A 和 DWL2/WOX9C 在顶端-基部轴的形成和干细胞的启动中都起着重要作用。此外，DWT1 在调控胚胎细胞最初几次分裂以确保胚胎在卵巢中的正确方向方面具有独特的功能。总之，DWT1 在水稻胚胎模式形成过程中作用于两个步骤：最初的合子分裂，以及授粉后 2 至 3 d 与 DWL2 一起建立主体轴和干细胞命运。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Plant Physiology 生物-植物科学

CiteScore

12.20

自引率

5.40%

发文量

535

审稿时长

2.3 months

期刊介绍： Plant Physiology® is a distinguished and highly respected journal with a rich history dating back to its establishment in 1926. It stands as a leading international publication in the field of plant biology, covering a comprehensive range of topics from the molecular and structural aspects of plant life to systems biology and ecophysiology. Recognized as the most highly cited journal in plant sciences, Plant Physiology® is a testament to its commitment to excellence and the dissemination of groundbreaking research. As the official publication of the American Society of Plant Biologists, Plant Physiology® upholds rigorous peer-review standards, ensuring that the scientific community receives the highest quality research. The journal releases 12 issues annually, providing a steady stream of new findings and insights to its readership.