EPB41L3 Inhibits the Progression of Cervical Cancer Via the ERK/p38 MAPK Signaling Pathway.

IF 2.4 4区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Molecular Biotechnology Pub Date : 2025-05-01 Epub Date: 2024-06-21 DOI:10.1007/s12033-024-01172-z

Gulixian Tuerxun, Wenyun Li, Guligeina Abudurexiti, Qian Zhuo, Awahan Tuerdi, Guzalinuer Abulizi

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Abstract

This study was aimed to uncover the character and potential regulatory mechanism of EPB41L3 in cervical cancer (CC). CC cells were injected into BALB/c nude mice (female) to construct a xenograft tumor model. Real-time quantitative polymerase chain reaction (qRT-PCR) and western blot were performed to evaluate the expression of EPB41L3, ERK/p38 MAPK signal markers in CC tissues and cells. Cell counting kit-8 (CCK-8) and Transwell was applied to analyze the viability, invasion, and migration of CC cell lines. EPB41L3 was substantially decreased both in CC tissues and cells. Cell viability, invasion, and migration of CC cells were reduced by overexpressing EPB41L3. Bioinformatics analysis prerdicted that EPB41L3 was strongly related to the ERK/p38 MAPK pathway. Compared with Ad-nc mice, the volume and weight of tumors and ERK/p38 MAPK signal markers were down-regulated in Ad-EPB41L3 mice. After knocking down EPB41L3 with EPB41L3 siRNA (siEPB41L3), the ERK/p38 MAPK pathway was activated. Moreover, SB203580 treatment reversed the effect of EPB41L3 silencing on the improvement in viability, migration, and invasion of CC cells. EPB41L3 suppresses the progression of CC via activating the ERK/p38 MAPK pathway. EPB41L3 may serve as an effective therapeutic target for CC.

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EPB41L3 通过 ERK/p38 MAPK 信号通路抑制宫颈癌的进展

本研究旨在揭示EPB41L3在宫颈癌（CC）中的特性和潜在调控机制。将 CC 细胞注射到 BALB/c 裸鼠（雌性）体内，构建异种移植肿瘤模型。采用实时定量聚合酶链反应（qRT-PCR）和免疫印迹法评估 EPB41L3、ERK/p38 MAPK 信号标志物在 CC 组织和细胞中的表达。应用细胞计数试剂盒-8（CCK-8）和Transwell分析了CC细胞系的活力、侵袭和迁移。EPB41L3在CC组织和细胞中均显著下降。过表达 EPB41L3 会降低 CC 细胞的活力、侵袭和迁移。生物信息学分析表明，EPB41L3与ERK/p38 MAPK通路密切相关。与Ad-nc小鼠相比，Ad-EPB41L3小鼠的肿瘤体积和重量以及ERK/p38 MAPK信号标志物均下调。用 EPB41L3 siRNA（siEPB41L3）敲除 EPB41L3 后，ERK/p38 MAPK 通路被激活。此外，SB203580治疗逆转了EPB41L3沉默对CC细胞活力、迁移和侵袭改善的影响。EPB41L3 通过激活 ERK/p38 MAPK 通路抑制了 CC 的进展。EPB41L3可作为CC的有效治疗靶点。

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来源期刊

Molecular Biotechnology 医学-生化与分子生物学

CiteScore

4.10

自引率

3.80%

发文量

165

审稿时长

6 months

期刊介绍： Molecular Biotechnology publishes original research papers on the application of molecular biology to both basic and applied research in the field of biotechnology. Particular areas of interest include the following: stability and expression of cloned gene products, cell transformation, gene cloning systems and the production of recombinant proteins, protein purification and analysis, transgenic species, developmental biology, mutation analysis, the applications of DNA fingerprinting, RNA interference, and PCR technology, microarray technology, proteomics, mass spectrometry, bioinformatics, plant molecular biology, microbial genetics, gene probes and the diagnosis of disease, pharmaceutical and health care products, therapeutic agents, vaccines, gene targeting, gene therapy, stem cell technology and tissue engineering, antisense technology, protein engineering and enzyme technology, monoclonal antibodies, glycobiology and glycomics, and agricultural biotechnology.