Phytoconstituent-derived zingerone nanoparticles disrupt the cell adhesion mechanism and suppress cell motility in melanoma B16F10 cells

IF 4.1 2区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Li-Wen Chu , Jun-Yih Chen , Yun-Wen Chen , Shuchen Hsieh , Mei-Lang Kung
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引用次数: 0

Abstract

Combining phytochemicals and nanotechnology to improve the unfavorable innate properties of phytochemicals and develop them into potent nanomedicines to enhance antitumor efficacy has become a novel strategy for cancer chemoprevention. Melanoma is the most aggressive, metastatic, and deadly disease of the primary cutaneous neoplasms. In this study, we fabricated phytoconstituent-derived zingerone nanoparticles (NPs) and validated their effects on cell adhesion and motility in melanoma B16F10 cells. Our data indicated that zingerone NPs significantly induced cytotoxicity and anti-colony formation and inhibited cell migration and invasion. Moreover, zingerone NPs dramatically interfered with the cytoskeletal reorganization and markedly delayed the period of cell adhesion. Our results also revealed that zingerone NPs-mediated downregulation of MMPs (matrix metalloproteinases) activity is associated with inhibiting cell adhesion and motility. We further evaluated the effects of zingerone NPs on Src/FAK /Paxillin signaling, our data showed that zingerone NPs significantly inhibited the protein activities of Src, FAK, and Paxillin, indicating that they play important roles in zingerone NP-mediated anti-motility and anti-invasion in melanoma cells. Accordingly, the phytoconstituent-zingerone NPs can strengthen the inhibition of tumor growth, invasion, and metastasis in malignant melanoma. Altogether, these multi-pharmacological benefits of zingerone NPs will effectively achieve the purpose of melanoma prevention and invasion inhibition.

Abstract Image

源于植物成分的姜酮纳米颗粒能破坏细胞粘附机制并抑制黑色素瘤 B16F10 细胞的移动。
将植物化学物质与纳米技术结合起来,改善植物化学物质的不利先天特性,并将其开发成有效的纳米药物,以提高抗肿瘤功效,已成为一种新型的癌症化学预防策略。黑色素瘤是原发性皮肤肿瘤中最具侵袭性、转移性和致命性的疾病。在这项研究中,我们制作了由植物成分衍生的姜酮纳米颗粒(NPs),并验证了它们对黑色素瘤 B16F10 细胞的细胞粘附性和运动性的影响。我们的数据表明,姜酮纳米粒子能显著诱导细胞毒性和抗集落形成,并抑制细胞迁移和侵袭。此外,姜酮 NPs 还能显著干扰细胞骨架的重组,明显延迟细胞粘附期。我们的研究结果还发现,姜酮 NPs 介导的 MMPs(基质金属蛋白酶)活性下调与抑制细胞粘附和运动有关。我们进一步评估了姜酮奈米对Src/FAK/Paxillin信号转导的影响,数据显示姜酮奈米显著抑制了Src、FAK和Paxillin的蛋白活性,表明它们在姜酮奈米介导的黑色素瘤细胞抗移动性和抗侵袭中发挥了重要作用。因此,植物成分姜酮 NPs 可加强对恶性黑色素瘤的肿瘤生长、侵袭和转移的抑制作用。总之,姜酮 NPs 的这些多重药理作用将有效地达到预防和抑制黑色素瘤侵袭的目的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of biotechnology
Journal of biotechnology 工程技术-生物工程与应用微生物
CiteScore
8.90
自引率
2.40%
发文量
190
审稿时长
45 days
期刊介绍: The Journal of Biotechnology has an open access mirror journal, the Journal of Biotechnology: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review. The Journal provides a medium for the rapid publication of both full-length articles and short communications on novel and innovative aspects of biotechnology. The Journal will accept papers ranging from genetic or molecular biological positions to those covering biochemical, chemical or bioprocess engineering aspects as well as computer application of new software concepts, provided that in each case the material is directly relevant to biotechnological systems. Papers presenting information of a multidisciplinary nature that would not be suitable for publication in a journal devoted to a single discipline, are particularly welcome.
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