Multilocus genetic analysis of Trypanosoma cruzi supports non-domestic intrusion into domestic transmission in an endemic region of Colombia

IF 2 Q3 INFECTIOUS DISEASES

Parasite Epidemiology and Control Pub Date : 2024-06-18 DOI:10.1016/j.parepi.2024.e00364

Omar Cantillo-Barraza , Jeiczon Jaimes-Dueñez , Paula L. Marcet , Omar Triana-Chavez , Andrés Gómez-Palacio

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Abstract

Trypanosoma cruzi, the causative agent of Chagas disease, is primarily transmitted to humans by hematophagous bugs of the Triatominae subfamily. In the Colombian Caribbean region, particularly on Margarita Island, T. cruzi transmission is highly endemic and associated with vectors such as Triatoma maculata and Rhodnius pallescens. Additionally, T. cruzi-infected Didelphis marsupialis are commonly found in close proximity to human dwellings. Given the complex transmission dynamics involving various domestic and non-domestic hosts, this study aimed to analyze 145 T. cruzi clones from twelve strains isolated from T. maculata, R. pallescens, and D. marsupialis using spliced leader intergenic region (SL-IR) sequences and nine polymorphic microsatellite loci. The results indicate the presence of a single polymorphic T. cruzi population, suggesting sustained local transmission dynamics between triatomines adapted to A. butyracea forests and peridomestic areas inhabited by synanthropic mammal reservoir such as D. marsupialis. Notably, this population appears to lack substructure, highlighting the importance of adopting an alternative eco-health approach to complement traditional chemical vector control methods for more effective and sustainable interruption of transmission.

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克氏锥虫的多焦点遗传分析表明，在哥伦比亚的一个地方病流行地区，国内传播中存在非国内侵入现象

南美锥虫病的病原体--克鲁兹锥虫，主要通过三足虫亚科的噬血虫传播给人类。在哥伦比亚加勒比海地区，特别是在玛格丽塔岛，T. cruzi 的传播是高度地方性的，并与黄斑蝽（Triatoma maculata）和苍蝇蝽（Rhodnius pallescens）等传播媒介有关。此外，在人类住所附近也经常发现感染了克鲁兹睾吸虫的马苏皮里斯（Didelphis marsupialis）。鉴于涉及各种家养和非家养宿主的复杂传播动态，本研究旨在利用剪接领导基因间区（SL-IR）序列和九个多态性微卫星位点，分析从巨蜥、白枕虫和马苏比利斯分离出的十二个菌株中的 145 个 T. cruzi 克隆。结果表明，存在一个单一的多态性 T. cruzi 种群，这表明在适应丁香林的三蠹类动物与同种哺乳动物（如 D. marsupialis）栖息的近郊地区之间存在持续的本地传播动态。值得注意的是，该种群似乎缺乏亚结构，这突出了采用替代性生态保健方法的重要性，以补充传统的化学病媒控制方法，从而更有效、更可持续地阻断传播。

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来源期刊

Parasite Epidemiology and Control Medicine-Infectious Diseases

CiteScore

5.70

自引率

3.10%

发文量

审稿时长

17 weeks

期刊介绍： Parasite Epidemiology and Control is an Open Access journal. There is an increasing amount of research in the parasitology area that analyses the patterns, causes, and effects of health and disease conditions in defined populations. This epidemiology of parasite infectious diseases is predominantly studied in human populations but also spans other major hosts of parasitic infections and as such this journal will have a broad remit. We will focus on the major areas of epidemiological study including disease etiology, disease surveillance, drug resistance and geographical spread and screening, biomonitoring, and comparisons of treatment effects in clinical trials for both human and other animals. We will also look at the epidemiology and control of vector insects. The journal will also cover the use of geographic information systems (Epi-GIS) for epidemiological surveillance which is a rapidly growing area of research in infectious diseases. Molecular epidemiological approaches are also particularly encouraged.