Synthesis and characterization of Spirulina-mediated titanium dioxide nanoparticles: Antimicrobial activity against multidrug-resistant bacteria

IF 5.45 Q1 Physics and Astronomy

Nano-Structures & Nano-Objects Pub Date : 2024-06-20 DOI:10.1016/j.nanoso.2024.101225

A. Mathesh , Aruchamy Mohanprasanth , Muthupandian Saravanan

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引用次数: 0

Abstract

Nanotechnology, particularly the use of nanoparticles, has garnered significant interest due to their unique properties and diverse applications, notably in antimicrobial research. This study focuses on the synthesis of titanium dioxide (TiO₂) nanoparticles mediated by Spirulina using green synthesis methods and explores their antibacterial effectiveness against multidrug-resistant bacteria, including Methicillin Resistance Staphylococcus aeruginosa, Pseudomonas aeruginosa, E. coli, and Enterococcus faecalis. The synthesis process involved the reduction of a titanium precursor using Spirulina biomass extract. Various characterization techniques, such as UV analysis, SEM imaging, FTIR spectroscopy, EDX analysis, and XRD, were employed to assess the physicochemical properties of the synthesized TiO₂ nanoparticles. Results showed a prominent absorbance peak at 322 nm and a band gap energy of 3.850 eV. SEM imaging revealed spherical morphology with aggregation, while XRD analysis indicated 61.4 % crystallinity with anatase phase. FTIR spectroscopy identified functional groups present in the nanoparticles, and EDX analysis confirmed the presence of titanium and oxygen elements. The antibacterial efficacy of Spirulina-mediated TiO₂ nanoparticles was evaluated using the Agar well diffusion method against multidrug-resistant bacteria. The nanoparticles exhibited significant inhibitory zones of 22 ±3, 17±4, 11±2, and 15±3 nm at 80 μg/ml against MRSA, P. aeruginosa, E. coli, and E. faecalis, respectively. Minimal microbial inhibition was observed at concentrations of 3.906, 15.625, 15.625, and 31.25 μg/ml for MRSA, Pseudomonas aeruginosa, Enterococcus faecalis, and E. coli, respectively. The minimum bactericidal concentrations (MBC) were found to be 7.812, 31.25, 31.25, and 62.5 μg/ml for the respective bacteria. This study highlights the effectiveness of Spirulina-mediated TiO₂ nanoparticles against multidrug-resistant bacterial strains in a bactericidal mode of action. Further research is warranted to investigate the molecular interactions between TiO₂ nanoparticles and multidrug-resistant bacteria.

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螺旋藻介导的二氧化钛纳米粒子的合成与表征：针对耐多药细菌的抗菌活性

纳米技术，尤其是纳米粒子的使用，因其独特的性能和多样化的应用而备受关注，特别是在抗菌研究方面。本研究的重点是利用绿色合成方法，以螺旋藻为媒介合成二氧化钛（TiO2）纳米粒子，并探讨其对耐多药细菌（包括耐甲氧西林铜绿葡萄球菌、铜绿假单胞菌、大肠杆菌和粪肠球菌）的抗菌效果。合成过程包括使用螺旋藻生物质提取物还原钛前体。研究人员采用了多种表征技术，如紫外分析、扫描电镜成像、傅立叶变换红外光谱、EDX 分析和 XRD，来评估合成的 TiO2 纳米粒子的理化性质。结果显示，在 322 纳米处有一个突出的吸光峰，带隙能为 3.850 eV。扫描电子显微镜成像显示了具有聚集的球形形态，而 XRD 分析表明锐钛矿相的结晶度为 61.4%。傅立叶变换红外光谱分析确定了纳米颗粒中存在的官能团，而乙二胺四乙酸氧化物分析则证实了钛和氧元素的存在。采用琼脂井扩散法评估了螺旋藻介导的 TiO2 纳米粒子对耐多药细菌的抗菌效果。在 80 μg/ml 的浓度下，纳米颗粒对 MRSA、铜绿假单胞菌、大肠杆菌和粪大肠杆菌的抑制作用分别为 22±3、17±4、11±2 和 15±3 nm。浓度分别为 3.906、15.625、15.625 和 31.25 μg/ml 时，对 MRSA、铜绿假单胞菌、粪肠球菌和大肠杆菌的微生物抑制作用最小。对相应细菌的最低杀菌浓度（MBC）分别为 7.812、31.25、31.25 和 62.5 μg/ml。这项研究强调了螺旋藻介导的 TiO2 纳米粒子在杀菌作用模式下对耐多药细菌菌株的有效性。有必要进一步研究二氧化钛纳米粒子与耐多药细菌之间的分子相互作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Nano-Structures & Nano-Objects Physics and Astronomy-Condensed Matter Physics

CiteScore

9.20

自引率

0.00%

发文量

审稿时长

22 days

期刊介绍： Nano-Structures & Nano-Objects is a new journal devoted to all aspects of the synthesis and the properties of this new flourishing domain. The journal is devoted to novel architectures at the nano-level with an emphasis on new synthesis and characterization methods. The journal is focused on the objects rather than on their applications. However, the research for new applications of original nano-structures & nano-objects in various fields such as nano-electronics, energy conversion, catalysis, drug delivery and nano-medicine is also welcome. The scope of Nano-Structures & Nano-Objects involves: -Metal and alloy nanoparticles with complex nanostructures such as shape control, core-shell and dumbells -Oxide nanoparticles and nanostructures, with complex oxide/metal, oxide/surface and oxide /organic interfaces -Inorganic semi-conducting nanoparticles (quantum dots) with an emphasis on new phases, structures, shapes and complexity -Nanostructures involving molecular inorganic species such as nanoparticles of coordination compounds, molecular magnets, spin transition nanoparticles etc. or organic nano-objects, in particular for molecular electronics -Nanostructured materials such as nano-MOFs and nano-zeolites -Hetero-junctions between molecules and nano-objects, between different nano-objects & nanostructures or between nano-objects & nanostructures and surfaces -Methods of characterization specific of the nano size or adapted for the nano size such as X-ray and neutron scattering, light scattering, NMR, Raman, Plasmonics, near field microscopies, various TEM and SEM techniques, magnetic studies, etc .