Electrospun radially oriented berberine-PHBV nanofiber dressing patches for accelerating diabetic wound healing.

IF 5.6 1区 医学 Q1 MATERIALS SCIENCE, BIOMATERIALS
Regenerative Biomaterials Pub Date : 2024-06-04 eCollection Date: 2024-01-01 DOI:10.1093/rb/rbae063
Qiuyu Wang, Sai Zhang, Jiayi Jiang, Shaojuan Chen, Seeram Ramakrishna, Wenwen Zhao, Fan Yang, Shaohua Wu
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引用次数: 0

Abstract

A dressing patch made of radially oriented poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) nanofibers was successfully manufactured with a modified electrospinning strategy. The as-electrospun PHBV radially oriented nanofiber dressing patch exhibited uniform and bead-free nanofibrous morphology and innovative radially oriented arrangement, which was demonstrated to possess obviously improved mechanical property, increased surface hydrophilicity and enhanced biological properties compared to the PHBV nanofiber dressing patch control with traditionally randomly oriented pattern. Interestingly, it was found that the radially oriented pattern could induce the cell migration from the periphery to the center along the radially oriented nanofibers in a rapid manner. To further improve the biofunction of PHBV radially oriented nanofiber dressing patch, berberine (Beri, an isoquinoline alkaloid) with two different concentrations were encapsulated into PHBV nanofibers during electrospinning, which were found to present a sustained drug release behavior for nearly one month. Importantly, the addition of Beri could impart the dressing patch with excellent anti-inflammatory property by significantly inhibiting the secretion of pro-inflammatory factors of M1 macrophages, and also showed an additive influence on promoting the proliferation of human dermal fibroblasts (HDFs), as well as inhibiting the growth of E. coli, S. aureus and C. albicans, compared with the Beri-free dressing patch. In the animal studies, the electrospun PHBV radially oriented nanofiber dressing patch loading with high Beri content was found to obviously accelerate the healing process of diabetic mouse full-thickness skin wound with shortened healing time (100% wound closure rate after 18 days' treatment) and improved healing quality (improved collagen deposition, enhanced re-epithelialization and neovascularization and increased hair follicles). In all, this study reported an innovative therapeutic strategy integrating the excellent physical cues of electrospun PHBV radially oriented nanofiber dressing patch with the multiple biological cues of Beri for the effective treatment of hard-to-heal diabetic wounds.

用于加速糖尿病伤口愈合的电纺径向小檗碱-PHBV 纳米纤维敷料贴片。
通过改进的电纺策略,成功制备了一种由径向取向聚(3-羟基丁酸-3-羟基戊酸)(PHBV)纳米纤维制成的敷料贴片。与传统随机取向的 PHBV 纳米纤维敷料贴片相比,电纺丝后的 PHBV 辐射取向纳米纤维敷料贴片呈现出均匀无珠的纳米纤维形态和创新的辐射取向排列,与传统随机取向的 PHBV 纳米纤维敷料贴片相比,具有明显改善的机械性能、增加的表面亲水性和增强的生物特性。有趣的是,研究还发现径向取向的图案能诱导细胞沿着径向取向的纳米纤维从外围向中心快速迁移。为了进一步提高 PHBV 辐射导向纳米纤维敷料贴片的生物功能,研究人员在电纺丝过程中将两种不同浓度的小檗碱(Berberine,一种异喹啉生物碱)封装到 PHBV 纳米纤维中,结果发现这种纳米纤维能持续释放药物近一个月。重要的是,与不含贝里的敷料贴片相比,贝里的添加能显著抑制 M1 巨噬细胞促炎因子的分泌,从而赋予敷料贴片优异的抗炎特性,同时还能促进人真皮成纤维细胞(HDFs)的增殖,抑制大肠杆菌、金黄色葡萄球菌和白色念球菌的生长。在动物实验中发现,电纺 PHBV 辐射导向纳米纤维敷料贴片含有大量贝里成分,能明显加速糖尿病小鼠全厚皮肤伤口的愈合过程,缩短愈合时间(治疗 18 天后伤口闭合率达 100%),提高愈合质量(改善胶原沉积,增强再上皮化和新生血管形成,增加毛囊)。总之,本研究报告了一种创新的治疗策略,它将电纺 PHBV 辐射导向纳米纤维敷料贴片的优异物理特性与 Beri 的多种生物特性相结合,有效治疗了难以愈合的糖尿病伤口。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Regenerative Biomaterials
Regenerative Biomaterials Materials Science-Biomaterials
CiteScore
7.90
自引率
16.40%
发文量
92
审稿时长
10 weeks
期刊介绍: Regenerative Biomaterials is an international, interdisciplinary, peer-reviewed journal publishing the latest advances in biomaterials and regenerative medicine. The journal provides a forum for the publication of original research papers, reviews, clinical case reports, and commentaries on the topics relevant to the development of advanced regenerative biomaterials concerning novel regenerative technologies and therapeutic approaches for the regeneration and repair of damaged tissues and organs. The interactions of biomaterials with cells and tissue, especially with stem cells, will be of particular focus.
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