Movement of ACC oxidase 3 mRNA from seeds to flesh promotes fruit ripening in apple.

IF 17.1 1区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Molecular Plant Pub Date : 2024-08-05 Epub Date: 2024-06-19 DOI:10.1016/j.molp.2024.06.008
Ting Wang, Yi Zheng, Chen Xu, Yulin Deng, Xinyi Hao, Zicheng Chu, Ji Tian, Yi Wang, Xinzhong Zhang, Zhenhai Han, Ting Wu
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引用次数: 0

Abstract

Xenia, the phenomenon in which the pollen genotype directly affects the phenotypic characteristics of maternal tissues (i.e., fruit ripening), has applications in crop production and breeding. However, the underlying molecular mechanism has yet to be elucidated. Here, we investigated whether mobile mRNAs from the pollen affect the ripening and quality-related characteristics of the fruit using cross-pollination between distinct Malus domestica (apple) cultivars. We demonstrated that hundreds of mobile mRNAs originating from the seeds are delivered to the fruit. We found that the movement of one of these mRNAs, ACC oxidase 3 (MdACO3), is coordinated with fruit ripening. Salicylic acid treatment, which can cause plasmodesmal closure, blocks MdACO3 movement, indicating that MdACO3 transcripts may move through the plasmodesmata. To assess the role of mobile MdACO3 transcripts in apple fruit, we created MdACO3-GFP-expressing apple seeds using MdACO3-GFP-overexpressing pollen for pollination and showed that MdACO3 transcripts in the transgenic seeds move to the flesh, where they promote fruit ripening. Furthermore, we demonstrated that MdACO3 can be transported from the seeds to fruit in the fleshy-fruited species tomato and strawberry. These results underscore the potential of mobile mRNAs from seeds to influence fruit characteristics, providing an explanation for the xenia phenomenon. Notably, our findings highlight the feasibility of leveraging diverse pollen genomic resources, without resorting to genome editing, to improve fruit quality.

ACC 氧化酶 3 mRNA 从种子到果肉的移动促进了苹果果实的成熟。
花粉基因型直接影响母体组织的表型特征(即果实成熟)的 "雌雄同株 "现象在作物生产和育种中有着广泛的应用。然而,其潜在的分子机制仍有待阐明。在这里,我们利用苹果不同栽培品种间的异花授粉研究了花粉中的移动 mRNA 是否会影响果实的成熟和质量相关特性。我们证明,数百个源自种子的移动 mRNA 被传递到果实中。我们还发现,其中一种 mRNA(ACC 氧化酶 3 (MdACO3))的移动与果实成熟相协调。水杨酸处理可导致质膜关闭,但会阻止 MdACO3 的移动,这表明 MdACO3 转录本可能会通过质膜移动。为了评估移动的 MdACO3 转录本在苹果果实中的作用,我们利用过表达 MdACO3-GFP 的花粉授粉,制造了表达 MdACO3-GFP 的苹果种子,结果表明转基因种子中的 MdACO3 转录本会移动到果肉中,并在果肉中调节果实成熟。此外,我们还证明了 MdACO3 可以从番茄和草莓这两种多肉果实物种的种子运输到果实中。这些结果凸显了种子中移动的 mRNA 影响果实特性的潜力,为 "雌雄同株 "现象提供了解释。值得注意的是,我们的研究结果突显了利用不同的花粉基因组资源而不诉诸基因组编辑来提高果实品质的可行性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Molecular Plant
Molecular Plant 植物科学-生化与分子生物学
CiteScore
37.60
自引率
2.20%
发文量
1784
审稿时长
1 months
期刊介绍: Molecular Plant is dedicated to serving the plant science community by publishing novel and exciting findings with high significance in plant biology. The journal focuses broadly on cellular biology, physiology, biochemistry, molecular biology, genetics, development, plant-microbe interaction, genomics, bioinformatics, and molecular evolution. Molecular Plant publishes original research articles, reviews, Correspondence, and Spotlights on the most important developments in plant biology.
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