The function of the cytoplasmic dynein light chain PTKM23 in the transport of PTSMAD2 during spermatogenesis in Portunus trituberculatus†.

IF 4.3 3区材料科学 Q1 ENGINEERING, ELECTRICAL & ELECTRONIC

ACS Applied Electronic Materials Pub Date : 2024-10-14 DOI:10.1093/biolre/ioae098

Qiu-Meng Xiang, Le Chang, Jun-Quan Zhu, Chang-Kao Mu, Chun-Lin Wang, Cong-Cong Hou

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Abstract

Cytoplasmic dynein participates in transport functions and is essential in spermatogenesis. KM23 belongs to the dynein light chain family. The TGFβ signaling pathway is indispensable in spermatogenesis, and Smad2 is an important member of this pathway. We cloned PTKM23 and PTSMAD2 from Portunus trituberculatus and measured their expression during spermatogenesis. PTKM23 may be related to cell division, acrosome formation, and nuclear remodeling, and PTSMAD2 may participate in regulating the expression of genes related to spermatogenesis. We assessed the localization of PTKM23 with PTDHC and α-tubulin, and the results suggested that PTKM23 functions in intracellular transport during spermatogenesis. We knocked down PTKM23 in vivo, and the expression of p53, B-CATAENIN and CYCLIN B decreased significantly, further suggesting a role of PTKM23 in transport and cell division. The localization of PTDIC with α-tubulin and that of PTSMAD2 with PTDHC changed after PTKM23 knockdown. We transfected PTKM23 and PTSMAD2 into HEK-293 T cells and verified their colocalization. These results indicate that PTKM23 is involved in the assembly of cytoplasmic dynein and microtubules during spermatogenesis and that PTKM23 mediates the participation of cytoplasmic dynein in the transport of PTSMAD2 during spermatogenesis.

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细胞质动力蛋白轻链 PTKM23 在三疣梭子蟹精子发生过程中运输 PTSMAD2 的功能。

细胞质动力蛋白参与运输功能，在精子发生过程中至关重要。KM23 属于动力蛋白轻链家族。TGFβ信号通路在精子发生过程中不可或缺，而Smad2是该通路的重要成员。我们从三疣梭子蟹中克隆了PTKM23和PTSMAD2，并测定了它们在精子发生过程中的表达。PTKM23可能与细胞分裂、顶体形成和核重塑有关，而PTSMAD2可能参与调控精子发生相关基因的表达。我们评估了PTKM23与PTDHC和α-微管蛋白的定位，结果表明PTKM23在精子发生过程中具有细胞内运输功能。我们在体内敲除了PTKM23，结果发现p53、B-CATAENIN和CYCLIN B的表达明显下降，这进一步表明PTKM23在运输和细胞分裂中发挥作用。PTKM23敲除后，PTDIC与α-微管蛋白的定位和PTSMAD2与PTDHC的定位发生了变化。我们将 PTKM23 和 PTSMAD2 转染到 HEK-293 T 细胞中，并验证了它们的共定位。这些结果表明，PTKM23 参与了精子发生过程中细胞质动力蛋白和微管的组装，并且 PTKM23 在精子发生过程中介导细胞质动力蛋白参与 PTSMAD2 的运输。该研究为三疣梭子蟹的育种提供了分子生物学理论依据。

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来源期刊

ACS Applied Electronic Materials Multiple-

CiteScore

7.20

自引率

4.30%

发文量

567