Corallococcus caeni sp. nov., a novel myxobacterium isolated from activated sludge

IF 2.3 3区生物学 Q3 MICROBIOLOGY

Archives of Microbiology Pub Date : 2024-06-21 DOI:10.1007/s00203-024-04037-w

Shun Tomita, Ryosuke Nakai, Kyohei Kuroda, Hazuki Kurashita, Masashi Hatamoto, Takashi Yamaguchi, Takashi Narihiro

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Abstract

Two myxobacterial strains (KH5-1^T and NO1) were isolated from the activated sludge tanks treating municipal sewage wastewater in Japan. These strains were recognised as myxobacteria based on their phenotypic characteristics of swarming colonies and fruiting bodies. Phylogenetic analyses using the 16S rRNA gene revealed that strains KH5-1^T and NO1 were affiliated with the genus Corallococcus, with the closest neighbours being Corallococcus exercitus AB043A^T (99.77% and 99.84%, respectively). Genome comparisons using orthologous average nucleotide identity (orthoANI) and digital DNA-DNA hybridisation similarity (dDDH) with strains KH5-1^T and NO1 and their phylogenetically close relatives in Corallococcus spp. were below the thresholds. The major cellular fatty acids of strains KH5-1^T and NO1 were iso-C_15:0 (31.9%, 30.0%), summed feature 3 (comprising C_16:1ω7c and/or C₁_6:1ω6c) (20.2%, 17.7%), and iso-C_17:0 (12.1%, 14.8%), and the major respiratory quinone was found to be menaquinone (MK)-8. Based on the phenotypic, chemotaxonomic, and phylogenetic evidence, strains KH5-1^T and NO1 represent a new species in the genus Corallococcus, for which the proposed name is Corallococcus caeni sp. nov. The type strain is KH5-1^T (= NCIMB 15510^T = JCM 36609^T).

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Corallococcus caeni sp.

从日本处理城市污水的活性污泥池中分离出两株霉菌（KH5-1T 和 NO1）。这些菌株根据其蜂拥菌落和子实体的表型特征被认定为霉菌。利用 16S rRNA 基因进行的系统进化分析表明，KH5-1T 和 NO1 菌株隶属于珊瑚球菌属，其中最相近的是 Corallococcus exercitus AB043AT（分别为 99.77% 和 99.84%）。用正交平均核苷酸同一性（orthoANI）和数字 DNA-DNA 杂交相似性（dDDH）对 KH5-1T 和 NO1 与珊瑚球菌属的系统发育近亲进行基因组比较，结果均低于阈值。菌株 KH5-1T 和 NO1 的主要细胞脂肪酸是异-C15:0（31.9%，30.0%）、总特征 3（包括 C16:1ω7c和/或 C16:1ω6c）（20.2%，17.7%）和异-C17:0（12.1%，14.8%），主要呼吸醌为 Menaquinone (MK)-8。根据表型学、化学分类学和系统发生学证据，菌株 KH5-1T 和 NO1 代表珊瑚球菌属的一个新种，拟命名为珊瑚球菌 caeni sp.模式菌株为 KH5-1T（= NCIMB 15510T = JCM 36609T）。

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来源期刊

Archives of Microbiology 生物-微生物学

CiteScore

4.90

自引率

3.60%

发文量

601

审稿时长

3 months

期刊介绍： Research papers must make a significant and original contribution to microbiology and be of interest to a broad readership. The results of any experimental approach that meets these objectives are welcome, particularly biochemical, molecular genetic, physiological, and/or physical investigations into microbial cells and their interactions with their environments, including their eukaryotic hosts. Mini-reviews in areas of special topical interest and papers on medical microbiology, ecology and systematics, including description of novel taxa, are also published. Theoretical papers and those that report on the analysis or ''mining'' of data are acceptable in principle if new information, interpretations, or hypotheses emerge.