Phosphorylation modification reverses the transcriptional inhibitory activity of CsMYB4a in tea plants (Camellia sinensis)

IF 5.7 1区农林科学 Q1 HORTICULTURE

Horticultural Plant Journal Pub Date : 2024-05-31 DOI:10.1016/j.hpj.2023.12.009

Guoliang Ma, Yunyun Han, Tongtong Li, Tao Xia, Liping Gao

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引用次数: 0

Abstract

The members of the fourth subgroup of R2R3-MYB (Sg4 members) are well-known inhibitors of phenylpropanoid and lignin synthesis pathways. The C2 domain is closely related to the transcriptional inhibitory activity of Sg4 members. Phosphorylation modification enhances the transcriptional inhibitory activity of Sg4 members. Here, we identified a phosphorylation site on the C2 domain of CsMYB4a from tea plants (). A mitogen-activated protein kinase (MAPK), named CsMPK3-2, phosphorylated this site on the C2 domain of CsMYB4a. Further experiments revealed that phosphorylation of CsMYB4a weakened its ability to inhibit the gene expression of , , and 4CL in the phenylpropanoid pathway and activated the expression of transcription factor , maintaining the adaxial–abaxial polarity of the leaf. Knocking out in transgenic tobacco partially repaired the leaf wrinkling phenotype caused by . The C1 domain exhibited an activation function when the C2 domain of CsMYB4a was phosphorylated by CsMPK3-2, causing this reversal phenomenon. These results enrich our understanding of the regulatory diversity of Sg4 members.

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磷酸化修饰可逆转茶树 CsMYB4a 的转录抑制活性

R2R3-MYB 第四亚群成员（Sg4 成员）是众所周知的苯丙素和木质素合成途径抑制剂。C2 结构域与 Sg4 成员的转录抑制活性密切相关。磷酸化修饰增强了 Sg4 成员的转录抑制活性。在这里，我们在茶树 CsMYB4a 的 C2 结构域上发现了一个磷酸化位点（）。一种名为 CsMPK3-2 的丝裂原活化蛋白激酶（MAPK）将 CsMYB4a C2 结构域上的这个位点磷酸化。进一步的实验发现，CsMYB4a的磷酸化削弱了其抑制苯丙酮途径中Ⅴ、Ⅴ和4CL基因表达的能力，激活了转录因子Ⅴ的表达，维持了叶片的正反极性。在转基因烟草中敲除Ⅴ，可部分修复由Ⅴ引起的叶片皱缩表型。当CsMYB4a的C2结构域被CsMPK3-2磷酸化时，C1结构域表现出激活功能，导致了这种逆转现象。这些结果丰富了我们对 Sg4 成员调控多样性的理解。

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来源期刊

Horticultural Plant Journal Environmental Science-Ecology

CiteScore

9.60

自引率

14.00%

发文量

293

审稿时长

33 weeks

期刊介绍： Horticultural Plant Journal (HPJ) is an OPEN ACCESS international journal. HPJ publishes research related to all horticultural plants, including fruits, vegetables, ornamental plants, tea plants, and medicinal plants, etc. The journal covers all aspects of horticultural crop sciences, including germplasm resources, genetics and breeding, tillage and cultivation, physiology and biochemistry, ecology, genomics, biotechnology, plant protection, postharvest processing, etc. Article types include Original research papers, Reviews, and Short communications.