Expression characterization of miR-6826-3p in Pardosa pseudoannulata (Araneae: Lycosidae) under high temperature stress

Abstract

Pardosa pseudoannulata is key predator in rice ecosystems; it plays a crucial role in rice pest biological control, effectively managing major rice pests such as rice planthopper. At present, there are few related studies on spiders' microRNA (miRNA). Consequently, we initiate a study on miR-6826-3p in P. pseudoannulata in response to high temperatures. First, we identified a total of 686 target genes of miR-6826-3p, 439 target genes had annotated results in the database, primarily involved in transcription and metabolism functions. Subsequently, quantitative real-time PCR was employed to assess the relative expression levels of miR-6826-3p in P. pseudoannulata under different temperatures (31, 34, 37, 40°C) and various durations (1, 2, 4, 8, 12 h). The results revealed that compared to the control group (25°C), the relative expression level of miR-6826-3p in female spiders significantly increased under the same temperature and different duration stresses. The expression level of miR-6826-3p in male spiders showed an initial increase followed by a decrease trend, with relatively lower expression levels after 1 and 12 h at the same temperature conditions. Furthermore, under the same duration of stress, compared to the control group (25°C), the relative expression level of miR-6826-3p in female spiders significantly increased at different temperatures. These results demonstrate that P. pseudoannulata miR-6826-3p plays a significant role in response to high-temperature stress. The research findings can provide a basis for future in-depth studies on the miRNA functional of P. pseudoannulata, aiding in the elucidation of the regulatory mechanisms of P. pseudoannulata miRNAs in response to high-temperature environments.