{"title":"Increased expression levels of PDGF and VEGF magnify the wound healing potential facilitated by biogenic synthesis of silver nanoparticles","authors":"Chella Perumal Palanisamy , Sirilux Poompradub , Kanokwan Sansanaphongpricha , Selvaraj Jayaraman , Karthik Subramani , Faridah Sonsudin","doi":"10.1016/j.nanoso.2024.101236","DOIUrl":null,"url":null,"abstract":"<div><p>Plant extract mediated biogenic synthesis of silver nanoparticles (AgNPs) has garnered considerable attention in nanotechnology due to its promising wound healing properties. This eco-friendly and cost-effective approach utilizes natural sources, such as the ethyl acetate extract of <em>Nigella sativa</em> L. (<em>N. sativa</em>) seeds, as a reducing agent. In this study, AgNPs were synthesized biogenically using <em>N. sativa</em> extract, and their wound healing potential was systematically assessed. Several methods for characterization are employed, incorporating ultraviolet-visible (UV-Vis) spectroscopy, fourier-transform infrared (FTIR) spectrometry, X-ray diffraction (XRD), scanning electron microscope (SEM) and dynamic light scattering (DLS) analysis were utilized to confirm successful synthesis and provide insight into the chemical and physical properties of AgNPs. When compared to a control group, human keratinocytes treated with AgNPs exhibited significantly enhanced proliferation and migration. Additionally, AgNPs were observed to increase the expression of wound-healing factors, (such as platelet-derived growth factor (PDGF) and vascular endothelial growth factor (VEGF)) as evidenced by western blot analysis. As a potent and naturally derived medicine for wound healing, AgNPs synthesized using <em>N. sativa</em> seed extract (ethyl acetate extract) potentially utilize the PDGF and VEGF signaling pathways to induce their therapeutic effects. Nevertheless, additional research is necessary to clarify the underlying mechanisms and assess the long-term safety and efficacy of this environmentally friendly method for producing AgNPs, which demonstrate remarkable wound-healing capabilities.</p></div>","PeriodicalId":397,"journal":{"name":"Nano-Structures & Nano-Objects","volume":null,"pages":null},"PeriodicalIF":5.4500,"publicationDate":"2024-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Nano-Structures & Nano-Objects","FirstCategoryId":"1","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2352507X24001471","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"Physics and Astronomy","Score":null,"Total":0}
引用次数: 0
Abstract
Plant extract mediated biogenic synthesis of silver nanoparticles (AgNPs) has garnered considerable attention in nanotechnology due to its promising wound healing properties. This eco-friendly and cost-effective approach utilizes natural sources, such as the ethyl acetate extract of Nigella sativa L. (N. sativa) seeds, as a reducing agent. In this study, AgNPs were synthesized biogenically using N. sativa extract, and their wound healing potential was systematically assessed. Several methods for characterization are employed, incorporating ultraviolet-visible (UV-Vis) spectroscopy, fourier-transform infrared (FTIR) spectrometry, X-ray diffraction (XRD), scanning electron microscope (SEM) and dynamic light scattering (DLS) analysis were utilized to confirm successful synthesis and provide insight into the chemical and physical properties of AgNPs. When compared to a control group, human keratinocytes treated with AgNPs exhibited significantly enhanced proliferation and migration. Additionally, AgNPs were observed to increase the expression of wound-healing factors, (such as platelet-derived growth factor (PDGF) and vascular endothelial growth factor (VEGF)) as evidenced by western blot analysis. As a potent and naturally derived medicine for wound healing, AgNPs synthesized using N. sativa seed extract (ethyl acetate extract) potentially utilize the PDGF and VEGF signaling pathways to induce their therapeutic effects. Nevertheless, additional research is necessary to clarify the underlying mechanisms and assess the long-term safety and efficacy of this environmentally friendly method for producing AgNPs, which demonstrate remarkable wound-healing capabilities.
期刊介绍:
Nano-Structures & Nano-Objects is a new journal devoted to all aspects of the synthesis and the properties of this new flourishing domain. The journal is devoted to novel architectures at the nano-level with an emphasis on new synthesis and characterization methods. The journal is focused on the objects rather than on their applications. However, the research for new applications of original nano-structures & nano-objects in various fields such as nano-electronics, energy conversion, catalysis, drug delivery and nano-medicine is also welcome. The scope of Nano-Structures & Nano-Objects involves: -Metal and alloy nanoparticles with complex nanostructures such as shape control, core-shell and dumbells -Oxide nanoparticles and nanostructures, with complex oxide/metal, oxide/surface and oxide /organic interfaces -Inorganic semi-conducting nanoparticles (quantum dots) with an emphasis on new phases, structures, shapes and complexity -Nanostructures involving molecular inorganic species such as nanoparticles of coordination compounds, molecular magnets, spin transition nanoparticles etc. or organic nano-objects, in particular for molecular electronics -Nanostructured materials such as nano-MOFs and nano-zeolites -Hetero-junctions between molecules and nano-objects, between different nano-objects & nanostructures or between nano-objects & nanostructures and surfaces -Methods of characterization specific of the nano size or adapted for the nano size such as X-ray and neutron scattering, light scattering, NMR, Raman, Plasmonics, near field microscopies, various TEM and SEM techniques, magnetic studies, etc .