Effect of moxibustion at "Xinshu" (BL15) and "Feishu" (BL13) on expression of TRPV1 and CGRP in the myocardial tissue of chronic heart failure rats.

Q3 Medicine

针刺研究 Pub Date : 2024-06-25 DOI:10.13702/j.1000-0607.20230820

Ling Zhu, Bing Gao, Tian-Tian Gong, Pan Liu, Lan Li, Ran Xia, Jing Wang

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引用次数: 0

Abstract

Objectives: To observe the effect of moxibustion at "Xinshu" (BL15) and "Feishu" (BL13) on transient receptor potential vanilloid type 1(TRPV1), calcitonin gene-related peptide (CGRP), and serum interleukin-10 (IL-10) in the myocardial tissue of rats with chronic heart failure (CHF), so as to explore its underlying mechanisms in improvement of CHF.

Methods: Male SD rats were randomly divided into the normal, model, moxibustion, capsaicin, moxibustion + capsaicin, and moxibustion + solvent groups, with 10 rats in each group. The CHF model was established by permanent ligation of the anterior descending branch of the left coronary artery. Mild moxibustion was applied to bilateral BL13 and BL15 for 30 min once daily for 4 weeks. Rats in the capsaicin group were smeared with capsaicin in the acupoint area once a day for 4 weeks. For rats of the moxibustion + capsaicin and moxibustion + solvent groups, capsaicin and solvent were applied to the acupoint area before moxibustion for 4 weeks, respectively. The ejection fraction (EF) and left ventricular fractional shortening rate (FS) were examined by echocardiography. HE staining was used to observe the myecardial morphological structure. The mRNA and protein expression levels of TRPV1, CGRP and galectin-3 (Gal-3) in myocardial tissue were detected by real-time quantitative PCR and Western blot, respectively. The content of IL-10 in serum was detected by ELISA.

Results: After modeling, the pathological changes of myocardium (as cardiac muscle fiber disorder, inflammatory cell infiltration, etc.) were obvious, and the EF, FS, serum IL-10, protein and mRNA exspression of TRPV1 and CGRP were significantly decreased (P<0.01) in the model group compared with the normal group, while the protein and mRNA exspression of Gal-3 were significantly up-regulated (P<0.01). Following the interventions, the above-mentioned indexes were all reversed in moxibustion, capsaicin, and moxibustion + capsaicin groups (P<0.01), and the effect of moxibustion + capsaicin was the best (P<0.05, P<0.01).

Conclusions: Moxibustion can reduce myocardial injury and improve cardiac function in CHF rats, which may be related to its effects in up-regulating the expression of TRPV1 and CGRP, and down-regulating the expression of Gal-3 to alleviate myocardial fibrosis.

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艾灸 "心舒"（BL15）和 "肺舒"（BL13）对慢性心衰大鼠心肌组织中 TRPV1 和 CGRP 表达的影响

目的观察艾灸 "心舒"(BL15)和 "肺舒"(BL13)穴对慢性心力衰竭(CHF)大鼠心肌组织中瞬时受体电位类香草素1(TRPV1)、降钙素基因相关肽(CGRP)及血清白细胞介素-10(IL-10)的影响，探讨其改善CHF的内在机制：雄性 SD 大鼠随机分为正常组、模型组、艾灸组、辣椒素组、艾灸 + 辣椒素组和艾灸 + 溶剂组，每组 10 只。通过永久性结扎左冠状动脉前降支建立 CHF 模型。在双侧 BL13 和 BL15 处进行温和艾灸，每天一次，每次 30 分钟，持续 4 周。辣椒素组大鼠在穴位区域涂抹辣椒素，每天一次，连续4周。艾灸+辣椒素组和艾灸+溶剂组的大鼠在艾灸前分别在穴位部位涂抹辣椒素和溶剂，连续4周。超声心动图检查射血分数（EF）和左心室折返缩短率（FS）。HE 染色用于观察心肌形态结构。实时定量 PCR 和 Western 印迹法分别检测了 TRPV1、CGRP 和 galectin-3 (Gal-3) 在心肌组织中的 mRNA 和蛋白表达水平。血清中 IL-10 的含量通过 ELISA 检测：结果：建模后，心肌的病理改变（如心肌纤维紊乱、炎性细胞浸润等）明显，EF、FS、血清IL-10、TRPV1和CGRP的蛋白和mRNA表达均显著下降（PPPPP结论：艾灸可减轻心肌的病理改变（如心肌纤维紊乱、炎性细胞浸润等）：艾灸能减轻CHF大鼠心肌损伤，改善心功能，可能与艾灸上调TRPV1和CGRP的表达，下调Gal-3的表达，减轻心肌纤维化有关。

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来源期刊

针刺研究 Medicine-Medicine (all)

CiteScore

1.30

自引率

0.00%

发文量

期刊介绍： Acupuncture Research was founded in 1976. It is an acupuncture academic journal supervised by the State Administration of Traditional Chinese Medicine, co-sponsored by the Institute of Acupuncture of the China Academy of Chinese Medical Sciences and the Chinese Acupuncture Association. This journal is characterized by "basic experimental research as the main focus, taking into account clinical research and reporting". It is the only journal in my country that focuses on reporting the mechanism of action of acupuncture. The journal has been changed to a monthly journal since 2018, published on the 25th of each month, and printed in full color. The manuscript acceptance rate is about 10%, and provincial and above funded projects account for about 80% of the total published papers, reflecting the latest scientific research results in the acupuncture field and has a high academic level. Main columns: mechanism discussion, clinical research, acupuncture anesthesia, meridians and acupoints, theoretical discussion, ideas and methods, literature research, etc.