Innate immune receptors are differentially expressed in mice during experimental Schistosoma mansoni early infection.

IF 2.5 4区医学 Q2 PARASITOLOGY

Memorias do Instituto Oswaldo Cruz Pub Date : 2024-06-17 eCollection Date: 2024-01-01 DOI:10.1590/0074-02760240013

Janete Cunha Lima, Ramayana Morais de Medeiros Brito, Luanderson Cardoso Pereira, Nathalie de Sena Pereira, Manuela Sales Lima Nascimento, Alan Lane de Melo, Paulo Marcos Matta Guedes

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引用次数: 0

Abstract

Background: The impact of Schistosoma mansoni infection over the immune response and the mechanisms involved in pathogenesis are not yet completely understood.

Objectives: This study aimed to evaluate the expression of innate immune receptors in three distinct mouse lineages (BALB/c, C57BL/6 and Swiss) during experimental S. mansoni infection with LE strain.

Methods: The parasite burden, intestinal tissue oogram and presence of hepatic granulomas were evaluated at 7- and 12-weeks post infection (wpi). The mRNA expression for innate Toll-like receptors, Nod-like receptors, their adaptor molecules, and cytokines were determined at 2, 7 and 12 wpi in the hepatic tissue by real-time quantitative polymerase chain reaction (qPCR).

Findings: Swiss mice showed 100% of survival, had lower parasite burden and intestinal eggs, while infected BALB/c and C57BL/6 presented 80% and 90% of survival, respectively, higher parasite burden and intestinal eggs. The three mouse lineages displayed distinct patterns in the expression of innate immune receptors, their adaptor molecules and cytokines, at 2 and 7 wpi.

Main conclusions: Our results suggest that the pathogenesis of S. mansoni infection is related to a dynamic early activation of innate immunity receptors and cytokines important for the control of developing worms.

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实验性曼氏血吸虫早期感染期间小鼠体内先天性免疫受体的表达存在差异。

背景：曼氏血吸虫感染对免疫反应的影响以及发病机制尚不完全清楚：本研究旨在评估在实验性曼氏血吸虫感染LE株期间，先天性免疫受体在三种不同小鼠品系（BALB/c、C57BL/6和瑞士鼠）中的表达情况：方法：在感染后 7 周和 12 周（wpi）对寄生虫负荷、肠道组织 Oogram 和肝肉芽肿的存在进行评估。通过实时定量聚合酶链反应（qPCR）测定肝组织中先天性 Toll 样受体、Nod 样受体及其适配分子和细胞因子的 mRNA 表达：结果：瑞士小鼠存活率为 100%，寄生虫负荷和肠道虫卵较少；而受感染的 BALB/c 和 C57BL/6 小鼠存活率分别为 80% 和 90%，寄生虫负荷和肠道虫卵较多。在2和7 wpi时，三种小鼠品系的先天性免疫受体、其适配分子和细胞因子的表达显示出不同的模式：我们的研究结果表明，曼氏沙门氏菌感染的发病机制与先天性免疫受体和细胞因子的早期动态激活有关，而先天性免疫受体和细胞因子对控制发育中的蠕虫非常重要。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Memorias do Instituto Oswaldo Cruz 医学-寄生虫学

CiteScore

5.00

自引率

3.60%

发文量

审稿时长

3-8 weeks

期刊介绍： Memórias do Instituto Oswaldo Cruz is a journal specialized in microbes & their vectors causing human infections. This means that we accept manuscripts covering multidisciplinary approaches and findings in the basic aspects of infectious diseases, e.g. basic in research in prokariotes, eukaryotes, and/or virus. Articles must clearly show what is the main question to be answered, the hypothesis raised, and the contribution given by the study. Priority is given to manuscripts reporting novel mechanisms and general findings concerning the biology of human infectious prokariotes, eukariotes or virus. Papers reporting innovative methods for diagnostics or that advance the basic research with these infectious agents are also welcome. It is important to mention what we do not publish: veterinary infectious agents research, taxonomic analysis and re-description of species, epidemiological studies or surveys or case reports and data re-analysis. Manuscripts that fall in these cases or that are considered of low priority by the journal editorial board, will be returned to the author(s) for submission to another journal.