Enhancing the wound healing process through local injection of exosomes derived from blood serum: An in vitro and in vivo assessment

IF 3.4 3区 环境科学与生态学 Q3 CELL & TISSUE ENGINEERING
Mehdi Rasti , Amir Hossein Parniaei , Leila Dehghani , Salar Nasr Esfahani , Hossein Mirhendi , Vida Yazdani , Vajihe Azimian Zavareh
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引用次数: 0

Abstract

Introduction

The skin plays a crucial role as a protective barrier against external factors, but disruptions to its integrity can lead to wound formation and hinder the natural healing process. Scar formation and delayed wound healing present significant challenges in skin injury treatment. While alternative approaches such as skin substitutes and tissue engineering exist, they are often limited in accessibility and cost. Exosomes have emerged as a potential solution for wound healing due to their regenerative properties.

Methods

In this study, exosomes were isolated from human blood serum using a kit. The exosomes were characterized, and their effects on cell migration were assessed in vitro. Additionally, the wound healing capacity of exosomes was evaluated in vivo using a rat full-thickness wound model.

Results

Our in vitro findings revealed that exosomes significantly promoted cell migration. In vivo experiments demonstrated that the injection of exosomes at different areas of the wound accelerated the wound healing process, resulting in wound closure, collagen synthesis, vessel formation, and angiogenesis in the wound area. These results suggest that exosomes have a promising therapeutic potential for expediting wound healing and minimizing scar formation.

Conclusions

The findings of this study highlight the potential of exosomes as a novel approach for enhancing wound healing. Exosomes showed positive effects on both cell migration and wound closure in in vitro and in vivo studies, suggesting their potential use as a regenerative therapy for skin injuries. Further research is needed to fully understand the mechanisms underlying the beneficial effects of exosomes on wound healing and to optimize their application in clinical settings.

通过局部注射血清外泌体促进伤口愈合:体外和体内评估
导言皮肤作为抵御外界因素的保护屏障起着至关重要的作用,但破坏其完整性会导致伤口形成并阻碍自然愈合过程。疤痕形成和伤口延迟愈合是皮肤损伤治疗的重大挑战。虽然存在皮肤替代品和组织工程等替代方法,但它们往往在可及性和成本方面受到限制。外泌体因其再生特性而成为伤口愈合的潜在解决方案。本研究使用试剂盒从人血清中分离出外泌体,并对外泌体进行了特征描述,在体外评估了外泌体对细胞迁移的影响。结果我们的体外实验结果表明,外泌体可显著促进细胞迁移。体内实验表明,在伤口的不同部位注射外泌体可加速伤口愈合过程,使伤口闭合、胶原合成、血管形成和血管生成。这些结果表明,外泌体在加速伤口愈合和减少疤痕形成方面具有良好的治疗潜力。在体外和体内研究中,外泌体对细胞迁移和伤口闭合都有积极作用,这表明外泌体有可能用作皮肤损伤的再生疗法。要充分了解外泌体对伤口愈合的有益作用机制,并优化其在临床中的应用,还需要进一步的研究。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Regenerative Therapy
Regenerative Therapy Engineering-Biomedical Engineering
CiteScore
6.00
自引率
2.30%
发文量
106
审稿时长
49 days
期刊介绍: Regenerative Therapy is the official peer-reviewed online journal of the Japanese Society for Regenerative Medicine. Regenerative Therapy is a multidisciplinary journal that publishes original articles and reviews of basic research, clinical translation, industrial development, and regulatory issues focusing on stem cell biology, tissue engineering, and regenerative medicine.
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